Cardimap tablet is a polyherbal formulation manufactured by Maharishi Ayurveda and used as an antihypertensive. The Ayurvedic formulation contains Rauwolfia serpentina, Nardostachys jatamansi, Convolvulus pluricaulis, Bacopa monnieri, Piper longum, and hence contains five markers, namely, reserpine, lupeol, scopoletine, bacoside A and piperine. This paper reports the standardization of Cardimap tablet according to World Health Organization (WHO) guideline and also by high-performance thin-layer chromatography method for the presence of the markers reserpine, lupeol, scopoletine, bacoside A and piperine. Both marketed and lab preparation complied all parameters according to WHO guideline and each marker compound showed a linear relationship in the concentration range 1‒2 µg per spot for reserpine, 2.5‒4.5 µg per spot for scopoletine, 10–50 µg per spot for piperine, 12–24 µg per spot for bacoside A and 1‒5 µg per spot lupeol. The proposed method was found to be precise, specific, and accurate, with a recovery of 98‒102%, and hence can be used for the routine analysis of this formulation.

Cardimap片剂是Maharishi Ayurveda生产的多草药配方，可用作抗高血压药。阿育吠陀制剂含有萝芙木serpentina，匙叶甘松，旋pluricaulis，假马齿苋，荜茇，因此含有五个标记物，即利血平，羽扇豆油，东pole碱，椰油甙A和胡椒碱。本文报告了根据世界卫生组织（WHO）指南对Cardimap片剂进行的标准化，并且还通过高效薄层色谱法对了利血平，卢比欧尔，东pole碱，ba子苷A和胡椒碱的标记物的存在进行了报道。市售制剂和实验室制剂均符合WHO指南的所有参数，并且每种标记物化合物的浓度范围为：利血平为每点1‒2 µg，东pole碱为每点2.5 µ4.5 µg，胡椒碱为每点10–50 µg线性关系，每份中的椰油甙A为12–24 µg，每份卢比欧酚为1‒5 µg。结果表明，所提方法准确，特异，准确，回收率为98％〜102％，可用于该配方的常规分析。