A highly reproducible plant regeneration protocol through somatic embryogenesis and shoot organogenesis has been developed for Cenchrus ciliaris. Three explants (seeds, shoot apices, and immature inflorescences) of four genotypes (IG-3108, IG-718, IG-74, and DBC15-8/32/10) were used for callus induction and plant regeneration. The highest rate of callus formation was found using Murashige and Skoog (MS) medium supplemented with 0.5 mg L⁻¹ benzylaminopurine (BA) and 3.0 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D). The largest number of somatic embryos was generated with the addition of 400 mg L⁻¹ L-proline, 400 mg L⁻¹ L-glutamine, and 300 mg L⁻¹ casein hydrolysate. Somatic embryos were successfully germinated on MS medium with 3.0 mg L⁻¹ BA and 0.25 mg L⁻¹ 2,4-D. In vitro plant regeneration was accomplished through somatic embryogenesis using all three explants. Ultra-structural features of somatic embryos confirmed proper formation and ontogeny.

通过体细胞胚胎发生和芽器官发生的高度可重现的植物再生方案已被开发用于ench。四种基因型（IG-3108，IG-718，IG-74和DBC15-8 / 32/10）的三种外植体（种子，芽尖和未成熟的花序）用于愈伤组织的诱导和植物再生。使用补充有0.5 mg L ^-1苄基氨基嘌呤（BA）和3.0 mg L ^-1 2,4-二氯苯氧基乙酸（2,4-D）的Murashige和Skoog（MS）培养基发现愈伤组织形成的最高速率。加入400 mg L ^-1 L脯氨酸，400 mg L ^-1 L谷氨酰胺和300 mg L ^-1产生最多的体细胞胚酪蛋白水解物。体细胞胚在含有3.0 mg L ^-1 BA和0.25 mg L ^-1 2,4-D的MS培养基上成功发芽。使用所有三种外植体通过体细胞胚发生完成了体外植物再生。体细胞胚的超微结构特征证实了适当的形成和个体发育。