Studies of chromosomes of Cyrtodactylus jarujini Ulber, 1993 and C. doisuthep Kunya et al., 2014 to compare microsatellite and TTAGGG sequences by classical and molecular techniques were conducted in Thailand. Karyological typing from a conventional staining technique of C. jarujini and C. doisuthep showed diploid chromosome numbers of 40 and 34 while the Fundamental Numbers (NF) were 56 in both species. In addition, we created the chromosome formula of the chromosomes of C. jarujini showing that 2n (40) = Lsm1 + Lsm2 + Lt3 + Mm1 + Mt4 + Sm2 + Sa2 + St5 while that of C. doisuthep was 2n (34) = Lsm3 + Lm2 + Lt3 + Mm1 + Mt2 + Sm4 + Sa1 + St1. Ag-NOR staining revealed NOR-bearing chromosomes in chromosome pairs 13 and 14 in C. jarujini, and in chromosome pairs 9 and 13 in C. doisuthep. This molecular study used the FISH technique, as well as microsatellite probes including (A)20, (TA)15, (CGG)10, (CGG)10, (GAA)10, (TA)15 and TTAGGG repeats. The signals showed that the different patterns in each chromosome of the Gekkonids depended on probe types. TTAGGG repeats showed high distribution on centromere and telomere regions, while (A)20, (TA)15, (CGG)10, (CGG)10, (GAA)10 and (TA)15 bearing dispersed over the whole genomes including chromosomes and some had strong signals on only a pair of homologous chromosomes. These results suggest that the genetic linkages have been highly differentiated between the two species.

中文翻译：

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来自泰国的两种壁虎物种（Reptilia，Gekkonidae）中微卫星和端粒重复序列的细胞遗传学表征和染色体作图

在泰国进行了研究，对Cyrtodactylus jarujini Ulber（1993年）和C.doisuthep Kunya等人（2014年）的染色体进行了比较，以通过经典和分子技术比较微卫星和TTAGGG序列。从传统的C. jarujini和C. doisuthep染色技术进行的核型分型显示两个物种的二倍体染色体数分别为40和34，而基本数（NF）为56。此外，我们创建了恶臭假单胞菌染色体的染色体公式，显示2n（40）= Lsm1 + Lsm2 + Lt3 + Mm1 + Mt4 + Sm2 + Sa2 + St5，而杜氏假丝酵母的2n（34）= Lsm3 + Lm2 + Lt3 + Mm1 + Mt2 + Sm4 + Sa1 + St1。Ag-NOR染色揭示了在假山梭菌中第13和14对染色体以及在杜氏梭菌中第9和13对染色体中带有NOR的染色体。这项分子研究使用了FISH技术，以及包括（A）20，（TA）15，（CGG）10，（CGG）10，（GAA）10，（TA）15和TTAGGG重复序列的微卫星探针。这些信号表明，Gekkonids每个染色体的不同模式取决于探针类型。TTAGGG重复序列显示着丝粒和端粒区域的高度分布，而（A）20，（TA）15，（CGG）10，（CGG）10，（GAA）10和（TA）15分布在整个基因组上，包括染色体和一些仅在一对同源染色体上具有强信号。这些结果表明，遗传联系已在两个物种之间高度区分。TTAGGG重复序列显示着丝粒和端粒区域的高度分布，而（A）20，（TA）15，（CGG）10，（CGG）10，（GAA）10和（TA）15分布在整个基因组上，包括染色体和一些仅在一对同源染色体上具有强信号。这些结果表明，遗传联系已在两个物种之间高度区分。TTAGGG重复序列显示着丝粒和端粒区域的高度分布，而（A）20，（TA）15，（CGG）10，（CGG）10，（GAA）10和（TA）15分布在整个基因组上，包括染色体和一些仅在一对同源染色体上具有强信号。这些结果表明，遗传联系已在两个物种之间高度区分。