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A highly-enhanced electrochemiluminescence luminophore generated by a metal–organic framework-linked perylene derivative and its application for ractopamine assay
Analyst ( IF 3.6 ) Pub Date : 2020-12-15 , DOI: 10.1039/d0an02186e Lijun Zhou 1, 2, 3, 4, 5 , Ding Jiang 1, 2, 3, 4, 5 , Yuru Wang 1, 2, 3, 4, 5 , Haibo Li 1, 2, 3, 4, 5 , Xueling Shan 1, 2, 3, 4, 5 , Wenchang Wang 1, 2, 3, 4, 5 , Zhidong Chen 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2020-12-15 , DOI: 10.1039/d0an02186e Lijun Zhou 1, 2, 3, 4, 5 , Ding Jiang 1, 2, 3, 4, 5 , Yuru Wang 1, 2, 3, 4, 5 , Haibo Li 1, 2, 3, 4, 5 , Xueling Shan 1, 2, 3, 4, 5 , Wenchang Wang 1, 2, 3, 4, 5 , Zhidong Chen 1, 2, 3, 4, 5
Affiliation
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In this study, a sensitive and effective monitoring method for ractopamine (RAC) was developed based on a sensitive electrochemiluminescence (ECL) aptasensor. Here, we employed a perylene derivative (PTC-PEI) with a Cu-based metal–organic framework (HKUST-1), which could accelerate the electron-transfer (ET) rate and strengthen interactions by the amido bond, resulting in enhanced ECL sensitivity and stability. Astonishingly, compared with the response of PTC-PEI and complex, the ECL signal of the MOF-based ECL material was noticeably raised by 6 times higher than that of PTC-PEI. HKUST-1 exhibited an excellent catalytic effect towards the electrochemical reduction process of S2O82−, thus allowing more sulfate radical anions (SO4˙−) to be generated. The strong ECL intensity of HKUST-1/PTC-PEI not only stemmed from the fixation of PTC-PEI that utilized its excellent film-forming abilities but also originated from the high porosity of HKUST-1 that carried more luminophores able to be excited. Satisfyingly, in the presence of the target molecule RAC, we observed an obvious quenching effect of signal, which could be attributed to aptamer recognition resulting in RAC being specifically captured on the electrode. Under optimal conditions, the developed sensor for the RAC assay displayed a desired linear range of 1.0 × 10−12–1.0 × 10−6 M and a low detection limit of 6.17 × 10−13 M (S/N = 3). This ECL sensor showed high sensitivity, good stability and excellent selectivity. More importantly, the proposed aptasensor exhibited excellent determination towards RAC detection and potential practical utility for real samples.
中文翻译：
由金属-有机骨架连接的per衍生物生成的高度增强的化学发光荧光团及其在莱克多巴胺测定中的应用
在这项研究中，基于灵敏的电化学发光（ECL）适体传感器，开发了一种灵敏有效的莱克多巴胺（RAC）监测方法。在这里，我们使用了具有铜基金属-有机骨架（HKUST-1）的per衍生物（PTC-PEI），它可以加快电子传递（ET）速率并通过酰胺键加强相互作用，从而增强ECL灵敏度和稳定性。令人惊讶的是，与PTC-PEI和复合物的响应相比，基于MOF的ECL材料的ECL信号明显提高了PTC-PEI的6倍。科大-1表现出优异的催化效果朝S的电化学还原处理2 ö 8 2-，从而使更多的硫酸根阴离子（SO 4 ˙ -）生成。HKUST-1 / PTC-PEI的强ECL强度不仅源于利用其出色的成膜能力的PTC-PEI的固定，还源于HKUST-1的高孔隙率，它携带了更多可激发的发光体。令人满意的是，在目标分子RAC的存在下，我们观察到了信号的明显淬灭作用，这可能归因于适体识别，导致RAC被特异性捕获在电极上。在最佳条件下，用于RAC分析的已开发传感器显示出1.0×10 -12 –1.0×10 -6 M的理想线性范围和6.17×10 -13的低检测限M（S / N = 3）。该ECL传感器显示出高灵敏度，良好的稳定性和出色的选择性。更重要的是，提出的适体传感器对RAC检测具有出色的测定能力，并且对实际样品具有潜在的实用性。
更新日期：2021-02-02
中文翻译：
由金属-有机骨架连接的per衍生物生成的高度增强的化学发光荧光团及其在莱克多巴胺测定中的应用
在这项研究中，基于灵敏的电化学发光（ECL）适体传感器，开发了一种灵敏有效的莱克多巴胺（RAC）监测方法。在这里，我们使用了具有铜基金属-有机骨架（HKUST-1）的per衍生物（PTC-PEI），它可以加快电子传递（ET）速率并通过酰胺键加强相互作用，从而增强ECL灵敏度和稳定性。令人惊讶的是，与PTC-PEI和复合物的响应相比，基于MOF的ECL材料的ECL信号明显提高了PTC-PEI的6倍。科大-1表现出优异的催化效果朝S的电化学还原处理2 ö 8 2-，从而使更多的硫酸根阴离子（SO 4 ˙ -）生成。HKUST-1 / PTC-PEI的强ECL强度不仅源于利用其出色的成膜能力的PTC-PEI的固定，还源于HKUST-1的高孔隙率，它携带了更多可激发的发光体。令人满意的是，在目标分子RAC的存在下，我们观察到了信号的明显淬灭作用，这可能归因于适体识别，导致RAC被特异性捕获在电极上。在最佳条件下，用于RAC分析的已开发传感器显示出1.0×10 -12 –1.0×10 -6 M的理想线性范围和6.17×10 -13的低检测限M（S / N = 3）。该ECL传感器显示出高灵敏度，良好的稳定性和出色的选择性。更重要的是，提出的适体传感器对RAC检测具有出色的测定能力，并且对实际样品具有潜在的实用性。
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