Seed conservation of banana crop wild relatives (Musa L. spp.) is limited because of lack of knowledge about their germination ecology. Musa acuminata Colla, the most important banana crop wild relative, is distributed in tropical and subtropical Asian and Pacific rainforests and colonizes disturbed sites. The role of temperature in stimulating/inhibiting germination to detect disturbance when canopy gaps are formed is not well known. We assessed seed germination thermal requirements of three subspecies of M. acuminata using nine seed accessions which had been stored in the Millennium Seed Bank. Diurnally alternating temperature cycles were almost completely essential for germination compared with constant temperatures. Germination was optimal when the upper temperature of a diurnal cycle was at 35°C; the lower temperature of the cycle was less important. Subspecies occurrence coordinates were used to extract climate temperature data which were then compared against the temperature requirements for germination from our experiment results. Maximum temperatures of the warmest month across subspecies ranges were close to but below optimal germination temperatures, as were diurnal ranges, suggesting soil-warming at the micro-climate level following gap creation is important for M. acuminata seed germination. Additionally, pre-treatment for 3 months at 60% relative humidity at constant 25°C improved germination from 14 ± 10 (mean, standard deviation) to 41 ± 29% suggesting a period in the soil seed bank under the canopy may increase sensitivity to alternating temperature cycles. Overall viability was low (49 ± 28%), and considerable variance was caused by the different accessions. Germination remained somewhat inconsistent.

中文翻译：

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在适应干扰的香蕉作物野生近缘种（Musa acuminata）中通过昼夜交替温度调节种子发芽

香蕉作物野生近缘种的种子保护（穆萨L. spp.) 由于缺乏对其萌发生态学的了解而受到限制。苦瓜Colla 是香蕉作物最重要的野生近缘种，分布在热带和亚热带的亚洲和太平洋热带雨林中，并在受干扰的地点定居。当冠层间隙形成时，温度在刺激/抑制发芽以检测干扰方面的作用尚不清楚。我们评估了三个亚种的种子发芽热需求M. acuminata使用存储在千年种子库中的九种种子种质。与恒温相比，昼夜交替的温度循环对于发芽几乎是完全必要的。当昼夜循环的上限温度为 35°C 时，发芽是最佳的；循环的较低温度不太重要。亚种发生坐标用于提取气候温度数据，然后将其与我们的实验结果中发芽的温度要求进行比较。整个亚种范围内最温暖月份的最高温度接近但低于最佳发芽温度，昼夜范围也是如此，这表明间隙形成后微气候水平的土壤变暖对于M. acuminata种子发芽。此外，在 60% 相对湿度和恒定 25°C 下进行 3 个月的预处理将发芽率从 14 ± 10（平均值，标准偏差）提高到 41 ± 29%，这表明树冠下土壤种子库中的一段时间可能会增加对交替的温度循环。总体生存力较低（49 ± 28%），不同种质造成了相当大的差异。发芽仍然有些不一致。