Granulosa cell tumour, an ovarian neoplasm of stromal origin, is an important tumour related to oestrogenic dominance syndrome and cystic endometrial hyperplasia–pyometra complex. In order to analyse ovarian tumour´s malignant potential, immunohistochemical markers can be used, such as anti‐HER2 and anti‐Ki‐67. The aim of this study was to evaluate the expression of immunohistochemical markers HER‐2 and Ki‐67 in granulosa cell tumour from bitches´ ovaries. In HER‐2 immunomarker analysis using the HercepTest^® method, most tumours were classified as 2+ (moderate labelling). Concerning Ki‐67 immunomarker, only one case was described as having a high proliferative index. An association was found between immunostained cell percentage by anti‐HER‐2 antibodies and high pleomorphism, represented by the pattern of follicular/trabecular tumour arrangement. There was no correlation between anti‐Ki‐67 and anti‐HER‐2 antibody immunostaining intensities, probably due to only one case with a high Ki‐67 index. With an effective protocol for HER‐2 and Ki‐67 immunohistochemical identification in granulosa cell tumours in bitches, it was possible to characterize this neoplasm proliferation profile.

中文翻译：

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HER-2和Ki-67在母犬颗粒细胞瘤中的免疫组化表达

颗粒细胞瘤是一种间质来源的卵巢肿瘤，是一种与雌激素优势综合征和囊性子宫内膜增生-子宫蓄脓复合体相关的重要肿瘤。为了分析卵巢肿瘤的恶性潜能，可以使用免疫组化标志物，如抗HER2和抗Ki-67。本研究的目的是评估免疫组织化学标志物 HER-2 和 Ki-67 在来自母犬卵巢的颗粒细胞瘤中的表达。使用 HercepTest ^{® 进行}HER-2 免疫标志物分析方法，大多数肿瘤被归类为 2+（中度标记）。关于 Ki-67 免疫标志物，只有一例被描述为具有高增殖指数。发现抗 HER-2 抗体免疫染色细胞百分比与高多形性之间存在关联，表现为滤泡/小梁肿瘤排列模式。抗 Ki-67 和抗 HER-2 抗体免疫染色强度之间没有相关性，可能是因为只有一个病例具有高 Ki-67 指数。通过在母犬颗粒细胞肿瘤中进行 HER-2 和 Ki-67 免疫组织化学鉴定的有效方案，可以表征这种肿瘤增殖特征。