A combination of allopurinol and benzbromarone is a common gout treatment protocol. A suboptimal response to allopurinol in patients is very common due to its pharmacokinetics variability. Moreover, the safe doses of benzbromarone is very crucial in patients with hepatic diseases. This raised the inquisitiveness to develop and optimize a capillary zone electrophoresis method for the determination of allopurinol and benzbromarone in their coformulation and in the presence of oxypurinol, the active metabolite of allopurinol, in biological and pharmaceutical matrices. The method greenness profile was assessed using green metric tools the “National Environmental Method Index,” the “Analytical Eco‐Scale,” and the “Green Analytical Procedure Index” by which the method proved to be ecofriendly. The method was successfully applied for the analysis of the pharmaceutical preparation and urine samples spiked with both drugs and the active metabolite. The linearity range was 25.0‐250.0 μg/mL for benzbromarone, 50.0‐350.0 μg/mL for allopurinol, and 100.0‐500.0 μg/mL for oxypurinol. The recoveries were 99.60 ± 0.67, 99.89 ± 0.98, and 98.71 ± 1.18% for benzbromarone, allopurinol, and oxypurinol, respectively. The analysis results indicate potential usefulness of capillary zone electrophoresis as a competitive and greener method of analysis in biological and quality control labs.

中文翻译：

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开发、优化和评估用于测定生物和药物基质中苯溴马隆和别嘌呤醇及其活性代谢物的绿色电泳方法

别嘌呤醇和苯溴马隆的组合是一种常见的痛风治疗方案。由于其药代动力学可变性，患者对别嘌呤醇的次优反应非常常见。此外，苯溴马隆的安全剂量对肝病患者非常重要。这提高了开发和优化毛细管区带电泳方法的好奇心，该方法用于在生物和药物基质中测定别嘌呤醇和苯溴马隆的共制剂中以及存在羟嘌呤醇（别嘌呤醇的活性代谢物）的情况。使用绿色度量工具“国家环境方法指数”、“分析生态量表”和“绿色分析程序指数”评估方法绿色度概况，通过这些工具证明该方法是生态友好的。该方法已成功应用于分析加标药物和活性代谢物的药物制剂和尿样。苯溴马隆的线性范围为 25.0-250.0 μg/mL，别嘌呤醇的线性范围为 50.0-350.0 μg/mL，羟嘌呤醇的线性范围为 100.0-500.0 μg/mL。苯溴马隆、别嘌呤醇和羟嘌呤醇的回收率分别为 99.60 ± 0.67、99.89 ± 0.98 和 98.71 ± 1.18%。分析结果表明毛细管区带电泳在生物和质量控制实验室中作为一种有竞争力和更环保的分析方法的潜在用途。苯溴马隆、别嘌呤醇和羟嘌呤醇分别为 98% 和 98.71 ± 1.18%。分析结果表明毛细管区带电泳在生物和质量控制实验室中作为一种有竞争力和更环保的分析方法的潜在用途。苯溴马隆、别嘌呤醇和羟嘌呤醇分别为 98% 和 98.71 ± 1.18%。分析结果表明毛细管区带电泳在生物和质量控制实验室中作为一种有竞争力和更环保的分析方法的潜在用途。