The fission yeast, Schizosaccharomyces pombe, is an excellent model for basic research but is not useful for commercial scale protein expression due to lack of strong expression vectors. Earlier, we showed that the lsd90 promoter elicited significantly greater GFP expression level than the adh1 and nmt1 promoters, albeit in different vector backbones. Here, we have systematically investigated the contribution of selectable markers, LEU2 and URA3m to GFP expression: while LEU2 elicited very low expression, the URA3m gene, with truncated promoter, elicited much greater GFP expression level with all promoters. Paradoxically, an inverse correlation was observed between the GFP transcription and translation efficiency. This system can be useful for understanding the factors governing recombinant gene expression and optimization of protein production.

裂殖酵母，粟酒裂殖酵母，是基础研究的极好模型，但由于缺乏强表达载体而不适用于商业规模的蛋白质表达。早些时候，我们表明lsd90启动子引发了比adh1和nmt1启动子显着更高的 GFP 表达水平，尽管在不同的载体骨架中。在这里，我们系统地研究了选择标记LEU2和URA3m对 GFP 表达的贡献：虽然LEU2引起非常低的表达，但URA3m具有截短启动子的基因，在所有启动子中都引发了更高的 GFP 表达水平。矛盾的是，在 GFP 转录和翻译效率之间观察到负相关。该系统可用于了解控制重组基因表达和优化蛋白质生产的因素。