Human bronchial epithelial (HBE) cells and c-fos-silenced HBE cells were first exposed to fine particulate matter (PM_2.5) and the resulting miRNA sequenced. Thereafter, a weighted gene co-expression network analysis was performed using Cytoscape software to visualize the interactions between identified hub miRNAs and their target genes. Nine differentially expressed miRNAs in hub miRNAs were identified in the different treatment groups, of which miR-25−3p, miR-215−5p, and miR-145−5p were selected for further study. Following qPCR validation, both miR-25−3p and miR-215−5p were found to be significantly up-regulated whilst, miR-145−5p was significantly down-regulated (p < 0.05) in the PM_2.5 group. Furthermore, miR-25−3p and miR-145−5p were also significantly down-regulated in the untreated group of c-fos silenced HBE cells. However, miR-215−5p was significantly down-regulated in both the untreated and PM_2.5-treated groups of c-fos silenced HBE cells. Subsequent analysis of their target genes also illustrated differential gene expression when comparing the treatment groups of the two cell types. The present data indicated that the c-fos gene has an important effect on the miRNA expression profiles and the related signaling pathways in PM_2.5-treated HBE cells. Therefore, each of miR-25−3p, miR-145−5p, and miR-215−5p may potentially provide future research information for additional exploration of a PM_2.5-induced carcinogenesis mechanism.

首先将人支气管上皮（HBE）细胞和c-fos沉默的HBE细胞暴露于细颗粒物（PM _2.5），并对产生的miRNA进行测序。之后，使用Cytoscape软件进行加权基因共表达网络分析，以可视化识别的轮毂miRNA与它们的靶基因之间的相互作用。在不同的治疗组中鉴定出了中枢miRNA中的九种差异表达的miRNA，其中miR-25-3p，miR-215-5p和miR-145-5p被选作进一步研究。经过qPCR验证后， 在PM _2.5中，发现miR-25-3p和miR-215-5p均显着上调，而miR-145-5p则显着下调（p <0.05）组。此外，在未经处理的c-fos沉默HBE细胞组中，miR-25-3p和miR-145-5p也显着下调。但是，在未经处理和经PM _2.5处理的c-fos沉默HBE细胞组中，miR-215-5p均显着下调。当比较两种细胞类型的治疗组时，其靶基因的后续分析也说明了差异基因的表达。本数据表明，c-fos基因对miRNA表达谱和在PM相关的信号传导途径的重要作用_2.5 -处理的HBE细胞。因此，miR-25-3p，miR-145-5p和miR-215-5p中的每一个都可能为进一步探索PM _2.5致癌机理提供潜在的研究信息。