Sclerotinia Stem Rot (SSR) caused by the oxalic acid (OA)-secreting necrotrophic fungal pathogen Sclerotinia sclerotiorum, causes significant yields losses in the crop Brassica sps. Oxalate oxidase (OxO) can metabolize OA to CO₂ and H₂O₂. Degradation of OA during the early phase of fungal–host interaction can interfere with the fungal infection and establishment processes. The present study demonstrates the potential of barley oxalate oxidase (BOxO) gene in conferring stable resistance against stem rot in a productive and highly susceptible Brassica juncea cv Varuna under field conditions. Four stable, independent, single-copy transgenic lines (B16, B17, B18, and B53) exhibited a significant reduction in the rate of lesion expansion i.e. 11–26%, 39–47%, and 24–35% reproducibly over the three-generation i.e. T₂, T₃, and T₄ respectively. The enhanced resistance in the transgenic lines correlated with high OxO activity, accumulation of higher levels of H₂O₂, and robust activation of defense responsive genes upon infection by S. sclerotiorum.

由分泌草酸 (OA) 的坏死性营养真菌病原体菌核病菌引起的菌核病茎腐病(SSR) 导致作物芸苔属植物的显着产量损失。草酸氧化酶 (OxO) 可以将 OA 代谢为 CO ₂和 H ₂ O ₂。在真菌-宿主相互作用的早期阶段，OA 的降解会干扰真菌感染和建立过程。本研究证明了大麦草酸氧化酶 ( BOxO ) 基因在赋予高产且高度易感的芥菜茎腐病稳定抗性方面的潜力。田间条件下的 cv Varuna。四个稳定的、独立的、单拷贝的转基因品系（B16、B17、B18 和 B53）表现出病变扩大率的显着降低，即 11-26%、39-47% 和 24-35% 可重复地超过三个- 代，即分别为 T ₂、T ₃和 T ₄。转基因品系中增强的抗性与高 OxO 活性、更高水平的 H ₂ O ₂积累以及在被核盘菌感染后防御反应基因的强烈激活相关。