The mitochondrial genome (mitogenome) of hybrid grouper Epinephelus moara (♀)×Epinephelus tukula (♂), a new hybrid progeny, can provide valuable information for analyzing phylogeny and molecular evolution. In this study, the mitogenome was analyzed using PCR amplification and sequenced, then the phylogenetic relationship of E. moara (♀)×E. tukula (♂) and 35 other species were constructed using Maximum Likelihood and Neighbor-Joining methods with the nucleotide sequences of 13 conserved protein-coding genes (PCGs). The complete mitogenome of E. moara (♀)×E. tukula (♂) was 16 695 bp in length, which contained 13 PCGs, 2 rRNA genes, 22 tRNA genes, a replication origin and a control region. The composition and order of these genes were consistent with most other vertebrates. Of the 13 PCGs, 12 PCGs were encoded on the heavy strand, and ND6 was encoded on the light strand. The mitogenome of the E. moara (♀)×E. tukula (♂) had a higher AT nucleotide content, a positive AT-skew and a negative GC-skew. All protein initiation codons were ATG, except for COX and ND4 (GTG), ATP6 (CTG), and ND3 (ATA). ND2, COXII, ND3, ND4 and Cytb had T as the terminating codon, COXIII’s termination codon was TA, and the remaining PCGs of that were TAA. All tRNA genes, except for the lacking DHU-arm of tRNA^Ser(AGN), were predicted to form a typical cloverleaf secondary structure. In addition, sequence similarity analysis (99% identity) and phylogenetic analysis (100% bootstrap value) indicated that the mitochondrial genome was maternally inherited. This study provides mitogenome data for studying genetic, phylogenetic relationships and breeding of grouper.

石斑鱼Epinephelus moara（♀）× Epinephelus tukula（♂）的杂交后代的线粒体基因组（mitogenome），可以为分析系统发育和分子进化提供有价值的信息。在这项研究中，有丝分裂基因组使用PCR扩增进行了分析和测序，然后进行了E. moara（♀）×E的系统发育关系。使用最大似然法和邻居加入法，用13个保守的蛋白质编码基因（PCG）的核苷酸序列构建了tukula（♂）和其他35个物种。E. moara（♀）×E的完整有丝分裂基因组。图库拉（♂）的长度为16 695 bp，包含13个PCG，2个rRNA基因，22个tRNA基因，复制起点和对照区。这些基因的组成和顺序与大多数其他脊椎动物一致。在13个PCG中，有12个PCG在重链上编码，而ND6在轻链上编码。所述的线粒体基因E. moara（♀）×E。tukula（♂）具有较高的AT核苷酸含量，正AT偏斜和负GC偏斜。除COX和ND4（GTG），ATP6（CTG）和ND3（ATA）外，所有蛋白质起始密码子均为ATG。ND2，COXII，ND3，ND4和Cytb以T作为终止密码子，COXIII的终止密码子为TA，其余PCG为TAA。所有tRNA基因，除了缺少tRNA ^{Ser （AGN）的}DHU臂，预计将形成一个典型的苜蓿叶形二级结构。此外，序列相似性分析（99％一致性）和系统发育分析（100％引导值）表明线粒体基因组是母系遗传的。该研究提供了有丝分裂基因组数据，用于研究石斑鱼的遗传，系统发育关系和繁殖。