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A graphene-based dengue immunosensor using plant-derived envelope glycoprotein domain III (EDIII) as the novel probe antigen
Analyst ( IF 3.6 ) Pub Date : 2021-2-1 , DOI: 10.1039/d0an02219e Qi Yan Siew 1, 2, 3, 4, 5 , Shi Hui Tan 2, 3, 4, 5, 6 , Ee Leen Pang 2, 3, 4, 7, 8 , Hwei-San Loh 2, 3, 4, 7, 8 , Michelle T. T. Tan 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2021-2-1 , DOI: 10.1039/d0an02219e Qi Yan Siew 1, 2, 3, 4, 5 , Shi Hui Tan 2, 3, 4, 5, 6 , Ee Leen Pang 2, 3, 4, 7, 8 , Hwei-San Loh 2, 3, 4, 7, 8 , Michelle T. T. Tan 1, 2, 3, 4, 5
Affiliation
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The envelope glycoprotein domain III (EDIII) of dengue virus (DENV) has been recognised as the antigenic region responsible for receptor binding. In the present work, we have proposed a novel immunosensor constructed on a graphene-coated screen-printed carbon electrode (SPCE) using plant-derived EDIII as the probe antigen to target DENV IgG antibodies. The developed immunosensor demonstrated high sensitivity towards DENV IgG within a wide linear working range (125–2000 ng mL−1) under the optimised sensing conditions. The limit of detection was determined to be 22.5 ng mL−1. The immunosensor also showed high specificity towards DENV IgG, capable of differentiating DENV IgG from the antibodies of other infectious diseases including the similarly structured Zika virus (ZIKV). The ability of the immunosensor to detect dengue antibodies in serum samples was also verified by conducting tests on mouse serum samples. The proposed immunosensor was able to provide a binary (positive/negative) response towards the serum samples comparable to the conventional enzyme-linked immunosorbent assay (ELISA), indicating promising potential for realistic applications.
中文翻译：
基于石墨烯的登革热免疫传感器，使用植物来源的包膜糖蛋白结构域III（EDIII）作为新型探针抗原
登革热病毒（DENV）的包膜糖蛋白结构域III（EDIII）被认为是负责受体结合的抗原区。在目前的工作中，我们提出了一种新的免疫传感器，该免疫传感器构建在石墨烯涂层的丝网印刷碳电极（SPCE）上，使用植物来源的EDIII作为探针抗原靶向DENV IgG抗体。在优化的传感条件下，已开发的免疫传感器在宽线性工作范围（125–2000 ng mL -1）中显示出对DENV IgG的高灵敏度。确定的检测下限为22.5 ng mL -1。免疫传感器还显示出对DENV IgG的高特异性，能够将DENV IgG与其他传染病的抗体区分开，包括类似结构的寨卡病毒（ZIKV）。免疫传感器检测血清样品中登革热抗体的能力也通过对小鼠血清样品进行测试来验证。所提出的免疫传感器能够对血清样品提供与常规酶联免疫吸附测定（ELISA）相媲美的二进制（阳性/阴性）响应，表明其在实际应用中具有广阔的前景。
更新日期：2021-02-01
中文翻译：
基于石墨烯的登革热免疫传感器，使用植物来源的包膜糖蛋白结构域III（EDIII）作为新型探针抗原
登革热病毒（DENV）的包膜糖蛋白结构域III（EDIII）被认为是负责受体结合的抗原区。在目前的工作中，我们提出了一种新的免疫传感器，该免疫传感器构建在石墨烯涂层的丝网印刷碳电极（SPCE）上，使用植物来源的EDIII作为探针抗原靶向DENV IgG抗体。在优化的传感条件下，已开发的免疫传感器在宽线性工作范围（125–2000 ng mL -1）中显示出对DENV IgG的高灵敏度。确定的检测下限为22.5 ng mL -1。免疫传感器还显示出对DENV IgG的高特异性，能够将DENV IgG与其他传染病的抗体区分开，包括类似结构的寨卡病毒（ZIKV）。免疫传感器检测血清样品中登革热抗体的能力也通过对小鼠血清样品进行测试来验证。所提出的免疫传感器能够对血清样品提供与常规酶联免疫吸附测定（ELISA）相媲美的二进制（阳性/阴性）响应，表明其在实际应用中具有广阔的前景。
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