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Hydrogen sulfide promotes flowering in heading Chinese cabbage by S-sulfhydration of BraFLCs
Horticulture Research ( IF 7.6 ) Pub Date : 2021-02-01 , DOI: 10.1038/s41438-020-00453-3 Xiaoli Ma , Liping Zhang , Zhuoya Pei , Linlin Zhang , Zhiqiang Liu , Danmei Liu , Xuefeng Hao , Zhuping Jin , Yanxi Pei
Horticulture Research ( IF 7.6 ) Pub Date : 2021-02-01 , DOI: 10.1038/s41438-020-00453-3 Xiaoli Ma , Liping Zhang , Zhuoya Pei , Linlin Zhang , Zhiqiang Liu , Danmei Liu , Xuefeng Hao , Zhuping Jin , Yanxi Pei
Heading Chinese cabbage (Brassica rapa L. syn.B .campestris L. ssp.chinensis Makino var.pekinensis (Rupr.) J. Cao et Sh. Cao) is a cruciferousBrassica vegetable that has a triplicate genome, owing to an ancient genome duplication event. It is unclear whether the duplicated homologs have conserved or diversified functions. Hydrogen sulfide (H2 S) is a plant gasotransmitter that plays important physiological roles in growth, development, and responses to environmental stresses. The modification of cysteines through S-sulfhydration is an important mechanism of H2 S, which regulates protein functions. H2 S promotes flowering inArabidopsis and heading Chinese cabbage. Here we investigated the molecular mechanisms of H2 S used to promote flowering in the latter. Four, five, and fourBraFLC ,BraSOC I , andBraFT homologs were identified in heading Chinese cabbage. Different BraFLC proteins were bound to different CArG boxes in the promoter regions of theBraSOC I andBraFT homologs, producing different binding patterns. Thus, there may be functionally diverseBraFLC homologs in heading Chinese cabbage. Exogenous H2 S at 100 μmol L−1 significantly promoted flowering by compensating for insufficient vernalization. BraFLC 1 and BraFLC 3 underwent S-sulfhydration by H2 S, after which their abilities to bind mostBraSOC I orBraFT promoter probes weakened or even disappeared. These changes in binding ability were consistent with the expression pattern of theBraFT andBraSOC I homologs in seedlings treated with H2 S. These results indicated that H2 S signaling regulates flowering time. In summary, H2 S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration.
更新日期：2021-02-01
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