Solanum lycopersicumvar.cerasiformeaccession PI 114490 has broad-spectrum resistance to bacterial spot caused by several species ofXanthomonas. Resistance is quantitatively inherited, and a common quantitative trait locusQTL-11Bon chromosome 11 has been identified previously. In this study, theSlPub24gene was characterized inQTL-11B.SlPub24in PI 114490 was upregulated by infection withX.euvesicatoriapv.perforansrace T3, but its transcription was low in the susceptible line OH 88119 whether or not it was infected by the pathogen. The differential expression ofSlPub24between PI 114490 and OH 88119 was due to great sequence variation in the promoter region. The promoter ofSlPub24in OH 88119 had very low activity and did not respond to pathogen infection. Transgenic lines of OH 88119 overexpressingSlPub24isolated from PI 114490 showed significantly enhanced resistance, while mutants ofSlpub24generated by CRISPR/Cas9 editing showed more susceptibility to race T3 and to other races. The mutants also showed spontaneous cell death in leaves. The expression of the salicylic acid (SA) pathway gene phenylalanine ammonia-lyase (PAL) and signaling-related genes pathogenesis-related (PR1)and nonexpresser ofPR1 (NPR1) were influenced bySlPub24. The content of SA in tomato plants was consistent with the level ofSlPub24expression. Furthermore, SlPUB24 interacted with the cell wall protein SlCWP and could regulate the degradation of SlCWP. The expression levels ofSlCWPandSlCWINV1, a cell wall invertase gene, showed opposite patterns during pathogen infection. The activity of SlCWINV1 was lower in mutants than in PI 114490. The results are discussed in terms of the roles of the abovementioned genes, and a potential model for SlPUB24-mediated resistance to bacterial spot is proposed.

中文翻译：

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番茄 SlPUB24 增强了对 Xanthomonas euvesicatoria pv. 的抗性。表演者比赛 T3

番茄变种。蜡茯苓加入 PI 114490 对由几种细菌引起的细菌斑点具有广谱抗性黄色单胞菌属. 抗性是数量遗传的，并且是一个共同的数量性状基因座QTL-11B先前已在 11 号染色体上发现。在这项研究中，SlPub24基因的特点是QTL-11B.SlPub24在 PI 114490 中被感染上调X.euvesicatoria光伏。穿孔器种族 T3，但其在易感品系 OH 88119 中的转录较低，无论它是否被病原体感染。的差异表达SlPub24PI 114490 和 OH 88119 之间的差异是由于启动子区域的巨大序列变异所致。发起人SlPub24OH 88119 的活性非常低，对病原体感染没有反应。过表达 OH 88119 的转基因系SlPub24从 PI 114490 中分离出的抗性显着增强，而 PI 114490 的突变体slpub24由 CRISPR/Cas9 编辑产生的结果显示出对 T3 种族和其他种族的敏感性更高。突变体还表现出叶子中的自发细胞死亡。水杨酸（SA）途径基因苯丙氨酸解氨酶的表达（朋友) 和信号相关基因发病机制相关 (公关1)并且不表达公关1 (NPR1) 受到影响SlPub24. 番茄植株中 SA 含量与SlPub24表达。此外，SlPUB24与细胞壁蛋白SlCWP相互作用，可以调节SlCWP的降解。的表达水平硅藻泥和SlCWINV1，一种细胞壁转化酶基因，在病原体感染期间表现出相反的模式。SlCWINV1在突变体中的活性低于PI 114490。从上述基因的作用角度讨论了结果，并提出了SlPUB24介导的细菌斑点抗性的潜在模型。