Trichomes that cover the epidermis of aerial plant organs play multiple roles in plant protection. Compared with a unicellular trichome in model plants, the development mechanism of the multicellular trichome is largely unclear. Notably, variations in trichome development are often accompanied by defects in the biosynthesis of cuticle and secondary metabolites; however, major questions about the interactions between developmental differences in trichomes and defects in metabolic pathways remain unanswered. Here, we characterized the glabrous mutantmict/csgl1/cstbhvia combined metabolomic and transcriptomic analyses to extend our limited knowledge regarding multicellular trichome development and metabolism in cucumber.Mictwas found to be explicitly expressed within trichome cells. Transcriptomic analysis indicated that genes involved in flavonoid and cuticle metabolism are significantly downregulated inmictmutants. Further metabolomic analysis confirmed that flavonoids, lipids, and cuticle compositions are dramatically altered inmictmutants. Additional studies revealed thatMictregulates flavonoid, lipid, and cuticle biosynthesis by likely directly binding to downstream functional genes, such asCsTT4,CsFLS1,CsCER26, andCsMYB36. These findings suggest that specific metabolic pathways (e.g., flavonoids and cuticle components) are co-regulated byMictand provide insights into transcriptional regulation mechanisms of multicellular trichome development and its specific metabolism in cucumber.

中文翻译：

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对微毛状体 (mict) 的研究揭示了黄瓜中多细胞毛状体发育的转录调控与特定代谢之间的新联系

覆盖气生植物器官表皮的毛状体在植物保护中发挥多重作用。与模式植物中的单细胞毛相比，多细胞毛的发育机制尚不清楚。值得注意的是，毛状体发育的变化通常伴随着角质层和次生代谢物生物合成的缺陷。然而，关于毛状体发育差异与代谢途径缺陷之间相互作用的主要问题仍未得到解答。在这里，我们表征了无毛突变体麦克风/csgl1/cstbh通过结合代谢组学和转录组学分析来扩展我们关于黄瓜多细胞毛状体发育和代谢的有限知识。麦克风被发现在毛细胞中明确表达。转录组学分析表明，参与类黄酮和角质层代谢的基因在麦克风突变体。进一步的代谢组学分析证实，黄酮类化合物、脂质和角质层成分在麦克风突变体。其他研究表明，麦克风通过可能直接与下游功能基因结合来调节类黄酮、脂质和角质层的生物合成，例如CsTT4,CsFLS1,CsCER26， 和CsMYB36. 这些发现表明，特定的代谢途径（例如，类黄酮和角质层成分）由麦克风并提供深入了解多细胞毛状体发育的转录调控机制及其在黄瓜中的特异性代谢。