ABSTRACT

• 1. The objective of this study was to evaluate performance of a commercial loop-mediated isothermal amplification (LAMP) method as an alternative method for the detection of Campylobacter spp. in primary production samples, poultry rinses and raw poultry products, as compared to the US Department of Agriculture Food Inspection Service Microbiology Laboratory Guide Book PCR reference method, MLG 41A.

• 2. The Campylobacter spp. LAMP was used in conjunction with a ready-to-use enrichment broth that does not require microaerophilic incubation. After enrichment, boot swabs from poultry farms, carcase rinses and raw poultry products were tested by the LAMP method and the MLG 41A PCR method.

• 3. The ready-to-use enrichment broth enabled the growth of Campylobacter spp. within 22 to 28 hours under aerobic incubation conditions. The LAMP method enabled Campylobacter detection in the enriched samples of various poultry matrices and had equivalent sensitivity and specificity to the MLG 41A PCR method.

• 4. No significant difference (95% confidence interval) was found between the alternative and the MLG 41A PCR method, as determined by probability of detection analysis, except for neutralising buffered peptone water post-chill rinsates. For the post-chill neutralising buffered peptone water rinsates, the LAMP method had significantly higher confirmed portions.

摘要

• 这项研究的目的是评估商业环路介导的等温扩增（LAMP）方法作为检测弯曲杆菌属的另一种方法的性能。与美国农业部食品检验局微生物学实验室指南书PCR参考方法MLG 41A相比，在初级生产样品，家禽漂洗液和未加工的家禽产品中。

• 2.弯曲杆菌属。LAMP与无需微需氧培养的即用型浓缩肉汤配合使用。浓缩后，通过LAMP方法和MLG 41A PCR方法测试了来自家禽场的靴子拭子，畜体冲洗液和未加工的家禽产品。

• 3.即用型浓缩肉汤可促进弯曲杆菌属的生长。在有氧培养条件下，在22至28小时内。LAMP方法可在各种家禽基质的富集样品中检测弯曲杆菌，并且与MLG 41A PCR方法具有同等的灵敏度和特异性。

• 4.通过中和检测分析的可能性确定，除了中和缓冲液中的蛋白ept水（冷却后漂洗液）外，其他方法与MLG 41A PCR方法之间没有发现显着差异（95％置信区间）。对于冷后中和的缓冲蛋白ept水冲洗液，LAMP方法的确证部分明显更高。