Abstract

Dodecyltrimethylammonium chloride (DTAC) was trapped into maltodextrins/pectin spray dried microcapsules to improve its activity against Salmonella enteritidis and Staphylococcus aureus biofilms. Two different microcapsules were prepared: uncomplexed DTAC-microcapsules (UDM), containing DTAC and maltodextrins; and complexed DTAC-microcapsules (CDM) containing DTAC complexed with pectin and maltodextrins. The minimum inhibitory concentrations (MIC) of both free and microencapsulated DTAC were investigated against S. Enteritidis and S. aureus. The MICs of DTAC were significantly lower when encapsulated. CDM treatment resulted in a 2 and 3.2 log reduction in S. aureus and S. Enteritidis biofilm culturable biomass, respectively. Microencapsulation reduced the cytotoxicity of DTAC by up to 32-fold. Free DTAC and CDM targeted the cell membrane resulting in the leakage of the intracellular molecules and subsequent cell death. The development of DTAC microcapsules reduced the amount of DTAC required to maintain the high standards of cleanliness and hygiene required in the food processing industries.

抽象的

将十二烷基三甲基氯化铵（DTAC）截留在麦芽糖糊精/果胶喷雾干燥的微胶囊中，以提高其对肠炎沙门氏菌和金黄色葡萄球菌生物膜的活性。制备了两种不同的微胶囊：未复合的DTAC微胶囊（UDM），其中含有DTAC和麦芽糖糊精；以及含有与果胶和麦芽糖糊精复合的DTAC的复合DTAC微胶囊（CDM）。游离和微胶囊化的DTAC的最低抑菌浓度（MIC）对进行了调查S.肠炎和金黄色葡萄球菌。封装时，DTAC的MIC显着降低。CDM处理导致金黄色葡萄球菌和链球菌的对数减少2和3.2个对数。肠炎生物膜可培养的生物质分别。微囊化最多可将DTAC的细胞毒性降低32倍。游离的DTAC和CDM靶向细胞膜，导致细胞内分子的泄漏和随后的细胞死亡。DTAC微胶囊的开发减少了维持食品加工行业所需的高标准清洁度和卫生性所需的DTAC量。