This study aimed to track the inheritance and identify the expressed sequence tag (EST) ‐ simple sequence repeat (SSR) marker (s) associated with the anthocyanin pigments in barnyard millet (Echinochloa spp.). The F₁ and F₂ generations were developed using the anthocyanin pigmented genotype ACM 331 (purple inflorescence) and three non‐pigmented genotypes, ACM 333, ACM 335 and MA 10 (green inflorescence). The F₁ plants of all the three crosses had medium purple inflorescence indicating that purple pigment was partially dominant over green inflorescence. The segregation ratio of F₂ generation is 1:2:1 (purple:medium purple:green) further confirmed that purple is partially dominant over the green and governed by one gene. Fifty‐one EST‐SSR markers were used to screen the pigmented and non‐pigmented bulks of the F₂ population. A marker, BMESSR 39 associated with the anthocyanin pigment trait was obtained, which revealed a high correlation with the anthocyanin pigment present in the F₂ population consisting of 430 individuals (85.10%) and 80 barnyard millet genotypes (63.75%). The information obtained in this study could pave way for breeding anthocyanin pigmented genotypes and genetic studies in barnyard millet.

中文翻译：

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bar中花色苷色素沉着的遗传学和分子标记（Echinochloa frumentacea（Roxb。）链接）

本研究旨在追踪遗传并鉴定与the（Echinochloa spp。）花色苷色素有关的表达序列标签（EST）-简单序列重复（SSR）标记。F ₁和F ₂代是用花青素着色基因型ACM 331（紫色花序）和三种非色素基因型ACM 333，ACM 335和MA 10（绿色花序）开发的。所有三个杂交的F ₁植物具有中等的紫色花序，表明紫色颜料部分地高于绿色花序。F ₂的偏析比世代为1：2：1（紫色：中等紫色：绿色）进一步证实了紫色在绿色部分占主导地位，并由一个基因控制。51个EST-SSR标记用于筛选F ₂种群的有色和无色块体。获得了与花青素色素性状相关的BMESSR 39标记，该标记表明与存在于由430个个体（85.10％）和80个bar基因型（63.75％）组成的F ₂种群中的花青素色素高度相关。这项研究获得的信息可为bar谷中花色苷色素的基因型育种和遗传研究铺平道路。