Host plant resistance is an effective means of managing aphid pests in safflower but its genetic basis is not known. In this study, F₁ and F₆‐recombinant inbred lines (RILs) produced from the CO‐1 × EC‐523368‐2 cross were phenotyped for tolerance to the aphid, Uroleucon compositae based on days‐to‐wilt (DW) after infestation. The F₁ plants showed the dominance effect and RILs exhibited quantitative variation. The RIL population was genotyped with 242 simple sequence repeat (SSR) markers, which included a set of 150 new SSRs designed through next‐generation sequencing and bioinformatics approaches. Two quantitative trait loci (QTLs), QUc‐Ct3.1 and QUc‐Ct5.1, putatively associated with DW after aphid infestation were detected. QUc‐Ct3.1 was a major QTL located on linkage group (LG) 3 with the closest marker SafM‐290 (LOD = 18.3), which explained 31.5% of the phenotypic variation. QUc‐Ct5.1 was a minor QTL located on LG‐5 with the closest marker CtDES‐237 (LOD = 7.0), which explained 9.1% of the phenotypic variation. This is the first report and a significant lead towards discovering genes for tolerance to aphids in safflower.

中文翻译：

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遗传图谱揭示了一个主要的QTL，其与对红花（Carthamus tinctorius）的蚜虫Uroleucon compositae（Theobald）的耐受性有关

寄主植物抗性是处理红花中蚜虫害虫的有效手段，但其遗传基础尚不清楚。在这项研究中，根据CO-1×EC‐523368‐2杂交产生的F ₁和F ₆重组自交系（RIL）的表型，根据其在枯萎后的天数（DW）对Uroleucon compositae蚜虫的耐受性侵扰。F ₁植物表现出显性效应，RILs表现出定量变化。RIL人群使用242个简单序列重复（SSR）标记进行基因分型，其中包括150个通过下一代测序和生物信息学方法设计的新SSR。两个数量性状基因座（QTL），QUc-Ct3。1和QUc‐Ct5.1，被认为与蚜虫侵染后的DW有关。QUc-Ct3。1是位于连锁群（LG）3上的主要QTL，具有最接近的标记SafM-290（LOD = 18.3），这解释了31.5％的表型变异。QUc-Ct5.1是位于LG-5上的次要QTL，带有最接近的标记CtDES-237（LOD = 7.0），这解释了9.1％的表型变异。这是第一份报告，也是发现红花蚜虫耐受性基因的重要线索。