Heterochiral tetrapeptide self-assembly into hydrogel biomaterials for hydrolase mimicry,Journal of Peptide Science

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Heterochiral tetrapeptide self-assembly into hydrogel biomaterials for hydrolase mimicry
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2021-01-31 , DOI: 10.1002/psc.3304
Marina Kurbasic ₁ , Ana M Garcia ₁ , Simone Viada ₁ , Silvia Marchesan ₁

Affiliation

Self-assembling short peptides have attracted great interest as enzyme mimics, especially if the catalytic activity resides solely in the supramolecular structure so that it can be switched on/off as needed by controlling assembly/disassembly. Among the various enzyme classes, hydrolases find wide application in biomaterials, and their mimetics often contain His residues, in addition to either divalent cations or other amino acids to mimic the catalytic site. This work reports two self-assembling tetrapeptides based on the Ser-His motif for catalysis and the Phe-Phe motif to drive amyloid structure formation. Both peptides form thermoreversible hydrogels in phosphate buffer at neutral pH that display a mild esterase-like activity, as demonstrated on the hydrolysis of 4-nitrophenyl acetate as a model substrate, although presence of Ser did not enhance catalytic activity. The systems are characterised by circular dichroism, transmission electron microscopy, oscillatory rheology and Thioflavin T fluorescence as an amyloid stain, to provide further insights that may assist the future design of improved supramolecular catalysts.

中文翻译：

异手性四肽自组装成水凝胶生物材料用于水解酶模拟

自组装短肽作为酶模拟物引起了极大的兴趣，特别是如果催化活性仅存在于超分子结构中，因此可以根据需要通过控制组装/拆卸来打开/关闭它。在各种酶类中，水解酶在生物材料中得到广泛应用，它们的模拟物通常含有 His 残基，此外还含有二价阳离子或其他氨基酸来模拟催化位点。这项工作报告了两种基于用于催化的 Ser-His 基序和用于驱动淀粉样蛋白结构形成的 Phe-Phe 基序的自组装四肽。两种肽在中性 pH 值的磷酸盐缓冲液中形成热可逆水凝胶，显示出温和的酯酶样活性，如作为模型底物的乙酸 4-硝基苯酯的水解所证明的那样，尽管 Ser 的存在并没有增强催化活性。该系统的特点是圆二色性、透射电子显微镜、振荡流变学和硫黄素 T 荧光作为淀粉样蛋白染色剂，以提供进一步的见解，有助于未来设计改进的超分子催化剂。

更新日期：2021-01-31

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