Bisphenol A (BPA) is a typical endocrine-disrupting chemical (EDC), which has been banned for food packing and baby ­ bottle material due to its potential endocrine harm to human body. In this paper, an easy and rapid immunochromatographic assay (ICA) combined with the technology of surface-enhanced Raman spectroscopy (SERS) was fabricated to improve the sensitivity and rapidity for quantitative determination of BPA. After 10 min of a quick chromatographic process, the specific Raman signal on T line of the test strip was measured. Compared with the traditional ICA, the SERS-ICA realized quantitative test and showed the limit of detection (LOD) was 0.1 ng/mL, getting approximately 300-fold lower. The method displayed high specificity and precision with RSD of 4.1%, and the test strip indicated favorable stability for two weeks without loss of activity. For river, tap and commercial drinking water sample, the SERS-ICA results were confirmed by HPLC, and the recoveries of spiked BPA were from 90.80-117.80%. It was shown that the proposed method takes advantages both of SERS and ICA, exhibiting high sensitivity, precision, stability, quantification and rapidity for field test, expecting a widely useful method for various small molecules in food safety, medical diagnosis and environmental samples.

双酚A（BPA）是一种典型的破坏内分泌的化学物质（EDC），由于其对人体的潜在内分泌危害，已被禁止用于食品包装和婴儿奶瓶材料。本文结合表面增强拉曼光谱技术（SERS）制备了一种快速简便的免疫色谱测定法（ICA），以提高双酚A定量测定的灵敏度和快速性。快速色谱过程10分钟后，测量试纸条T线上的特定拉曼信号。与传统ICA相比，SERS-ICA进行了定量测试，显示检测限（LOD）为0.1 ng / mL，降低了约300倍。该方法具有很高的特异性和精密度，相对标准偏差为4.1％，试纸在两周内显示出良好的稳定性，而没有失去活性。对于河流，自来水和商业饮用水样品，HPLC证实了SERS-ICA结果，加标BPA的回收率为90.80-117.80％。结果表明，该方法兼具SERS和ICA的优点，具有较高的灵敏度，精密度，稳定性，定量性和快速性，可望用于食品安全，医学诊断和环境样品中的各种小分子方法。