Four species belonging to the genus Euplotes have been investigated, namely: E. lynni nov. spec., E. indica nov. spec., E. aediculatus, and E. woodruffi. All populations are from India and were investigated using morphological and molecular markers. The phylogenetic relationships were inferred from small subunit ribosomal rRNA gene (SSU rRNA), internal transcribed spacer (ITS) region, and mitochondrial cytochrome c oxidase subunit I (COI) gene. Predicted secondary structure models for two new species using the hypervariable region of the SSU rRNA gene and ITS2 region support the distinctness of both species. Morphological characters were subjected to principal component analysis (PCA) and genetic variations were studied in-depth to analyze the relatedness of the two new species with their congeners. An integrative approach combining morphological features, molecular analysis, and ecological characteristics was carried out to understand the phylogenetic position of the reported species within the different clades of the genus Euplotes.

四种属游仆进行了研究，分别是：E。lynni十一月 spec。，E. indica nov。规格，E。aediculatus和E. woodruffi。所有种群均来自印度，并使用形态学和分子标记进行了调查。从小亚基核糖体rRNA基因（SSU rRNA），内部转录间隔区（ITS）区域和线粒体细胞色素C氧化酶亚基I（COI）基因推断出系统发育关系。使用SSU rRNA基因的高变区和ITS2区的两个新物种的预测二级结构模型支持这两个物种的独特性。对形态特征进行主成分分析（PCA），并深入研究了遗传变异，以分析两个新物种与其同类物的相关性。结合形态特征，分子分析，真人。