Nitrous oxide (N₂O), an important greenhouse gas, is emitted from landfill reservoirs, especially in the working face, where nitrification and denitrification occur under different O₂ concentrations. In order to explore the effects of O₂ concentration on N₂O emissions and production pathways, the production of N₂O from simulated fresh waste landfilling under 0%, 5%, 10%, and 21% (vol/vol) O₂ concentrations were examined, and ¹⁵N isotopes were used as tracers to determine the contributions of nitrification (NF), heterotrophic denitrification (HD), and nitrification-coupled denitrification (NCD) to N₂O production over a 72-h incubation period. Equal amounts of total nitrogen consumption occurred for all studied O₂ concentration and the simulated waste tended to release more N₂O under 0% and 21% O₂. Heterotrophic denitrification was the main source of N₂O release at the studied oxygen concentrations, contributing 90.51%, 69.04%, 80.75%, and 57.51% of N₂O under O₂ concentrations of 0%, 5%, 10%, and 21%, respectively. Only denitrification was observed in the simulated fresh waste when the oxygen concentration of the bulk atmosphere was 0%. The nitrate reductase (nirS)-encoding denitrifiers in the simulated landfill were also studied and significant differences were observed in the richness and diversity of the denitrifying community at different taxonomic levels. It was determined that optimising the O₂ content is a crucial factor in N₂O production that may allow greenhouse gas emissions and N turnover during landfill aeration to be minimised.

一氧化二氮（N ₂ O）是一种重要的温室气体，它从垃圾填埋场中释放出来，尤其是在工作面中，在不同的O ₂浓度下都会发生硝化和反硝化作用。为了探索的O的影响₂上N浓度_2个O排放和生产途径中，制备N ₂下0％从模拟新鲜垃圾填埋O，5％，10％，和21％（体积/体积）O ₂检查浓度，并使用¹⁵ N同位素作为示踪剂以确定硝化（NF），异养反硝化（HD）和硝化耦合反硝化（NCD）对N ₂的贡献在72小时的孵育时间内产生O。总氮消耗等量的发生对所有研究的Ò ₂浓度和模拟废物倾向于释放更多Ñ ₂下0％和21％○○ ₂。异养脱氮为：N的主要来源₂在所研究的氧浓度ø释放，贡献90.51％，69.04％，80.75％，以及N 57.51％₂下○○ ₂ 0％，5％，10％，21的浓度％， 分别。当整体气氛中的氧气浓度为0％时，在模拟的新鲜废物中仅观察到反硝化作用。硝酸还原酶（NIRS还研究了模拟垃圾填埋场中的（-）编码反硝化剂，并观察到了不同分类学级别的反硝化群落的丰富性和多样性差异。已确定优化O ₂含量是N ₂ O生产中的关键因素，可以使填埋曝气期间的温室气体排放量和N转换量降至最低。