当前位置: X-MOL 学术Braz. J. Bot. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The complete chloroplast genome of Prunus triloba var. plena and comparative analysis of Prunus species: genome structure, sequence divergence, and phylogenetic analysis
Brazilian Journal of Botany ( IF 1.4 ) Pub Date : 2021-02-01 , DOI: 10.1007/s40415-020-00685-6
Li Wang , Zhihong Guo , Qianhan Shang , Wei Sa , Le Wang

Prunus triloba var. plena, also known as flowering plum, is a perennial deciduous shrub admired for its brilliant pink-purple blooms. Chloroplast (cp) DNA is highly conserved in structure and gene arrangement, making cp genomic data valuable resources for species delimitation and phylogenetics. The cp genome of P. triloba var. plena was de novo assembled with the aim of developing cp-derived molecular markers and deepening the understanding of phylogenetic relationship among Prunus species. The complete cp genome was 158,022 bp in length, with a GC content of 36.8%. The plastome featured a typical quadripartite structure, consisting of a pair of inverted repeat (IR) regions of 26,379 bp, separated by a large single copy (LSC) region of 86,242 bp, and a small single copy (SSC) region of 19,022 bp. The cp genome encoded 134 genes, including 87 protein-coding genes, 39 tRNA genes, and 8 rRNA genes. A total of 59 simple sequence repeat (SSR) were identified, 68% of which were located in the intergenic regions. An obvious A/T bias was observed in the majority of SSRs detected. Besides, 48 repeats in different sizes and types were detected. These repeats, together with SSRs and the divergence hotspots detected, could serve as markers facilitating species discrimination and evolutionary research in Prunus. Using plastome sequences, we re-investigated the phylogenetic relationship among 32 Prunus species. These species explicitly clustered into three monophyletic clades, among which P. triloba var. plena was closely related to species in the subgenera Amygdalus and Prunus.



中文翻译:

樱桃李的完整叶绿体基因组。樱桃和李属物种的比较分析:基因组结构,序列差异和系统发育分析

榆叶梅变种。plena,也称为开花李,是一种多年生落叶灌木,因其艳丽的粉红色紫色花朵而备受赞赏。叶绿体(cp)DNA在结构和基因排列上高度保守,使cp基因组数据成为物种界定和系统发育研究的宝贵资源。P. triloba var的cp基因组。从头开始进行plena组装,目的是开发cp衍生的分子标记并加深对之间系统发生关系的了解种类。完整的cp基因组长度为158,022 bp,GC含量为36.8%。质体组具有典型的四方结构,由一对26,379 bp的反向重复(IR)区域组成,由一个86,242 bp的大单拷贝(LSC)区域和一个19,022 bp的小单拷贝(SSC)区域隔开。cp基因组编码134个基因,包括87个蛋白质编码基因,39个tRNA基因和8个rRNA基因。总共鉴定出59个简单序列重复序列(SSR),其中68%位于基因间区域。在大多数检测到的SSR中观察到明显的A / T偏差。此外,还检测到48个不同大小和类型的重复序列。这些重复序列与SSR和检测到的发散热点一起,可作为促进李属物种歧视和进化研究的标志。使用质体序列,我们重新调查了32个李属物种之间的系统发育关系。这些物种明确地分为三个单系进化枝,其中P. triloba var。重瓣密切相关的亚属种樱桃。

更新日期:2021-02-01
down
wechat
bug