The black truffle (Tuber melanosporum Vittad.) and the summer truffle (Tuber aestivum Vittad.) are two of the most appreciated edible fungi worldwide. The natural distributions of both species partially overlap. However, the interspecific interactions between these truffles and how irrigation and mulching techniques impact the dynamics between them are still unknown. Here, an experimental truffle plantation with Quercus ilex was established in Maials (Catalonia, Spain), combining three soil mulch treatments (white mulch, black mulch and bare soil as a control) and two irrigation regimes (irrigated and non-irrigated as a control) to investigate truffle mycelial dynamics in soil when both truffle species co-occur. The development of truffle mycelium in two different seasons (spring and autumn) in two consecutive years (2017 and 2018) was quantified using qPCR. Truffle mycelia of both species showed greatest development under white mulch. When mycelia of both truffle species co-occurred in soil, irrigation combined with white mulch resulted in greater quantities of T. melanosporum mycelial biomass, whereas the control irrigation treatment favoured the development of T. aestivum. Mulch treatments were also advantageous for seedling growth, which was expressed as root collar diameter and its increment during the study period. Significant relationships between root collar diameter and root growth and the amount of mycelial biomass in the soil were observed for both truffle species. Our results indicate the potential advantages of using white mulch to support irrigation in truffle plantations located in areas with dry Mediterranean climatic conditions to promote the development of Tuber mycelium.

黑松露菌（Tuber melanosporum Vittad。）和夏季松露菌（Tuber aestivum Vittad。）是全球最受欢迎的食用菌中的两种。两个物种的自然分布部分重叠。然而，这些松露之间的种间相互作用以及灌溉和覆盖技术如何影响它们之间的动力学仍然未知。在这里，用栎栎进行实验性松露种植在马亚尔（西班牙加泰罗尼亚）建立了三种土壤覆盖处理方法（白膜覆盖，黑膜覆盖和裸露土壤作为对照）和两种灌溉方式（灌溉和非灌溉作为对照），以研究松露菌在土壤中的菌丝动力学。两种松露菌同时出现。使用qPCR定量了连续两年（2017年和2018年）在两个不同季节（春季和秋季）的松露菌丝体的发育。两种覆盖物的松露菌丝体显示出最大的生长。当两种松露菌种的菌丝体同时存在于土壤中时，灌溉与白覆盖一起导致黑麦草的菌丝体生物量增加，而对照灌溉处理则有利于小麦的生长。。覆盖处理对幼苗生长也是有利的，这表示为根领直径及其在研究期间的增量。两种松露菌种的根围直径与根生长和土壤中菌丝体生物量之间均存在显着关系。我们的结果表明，在地中海气候干燥地区的松露种植园中，使用白覆盖物支持灌溉的潜在优势，可以促进块茎菌丝体的发育。