Resistance is induced in cotton plants as the result of either viral infection or exogenous application of elicitors. Induced resistance can be evaluated by determining the production of β-1,3-glucanase and chitinase in plants as a biochemical parameter. The assays being used for the determination of chitinase and β-1,3-glucanase activity are laborious and not cost-effective, as the reducing sugars produced by the substrates colloidal chitin and laminarin are very expensive. The concentration of both substrates was standardized and reduced to 0.25% from 4% in a modified microplate assay, which appeared to be more effective. The amount of β-1,3-glucanase and chitinase produced was significant and determined by the new modified assay. The sensitivity of the microplate assay was significantly raised approximately one- to twofold.

中文翻译：

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应用稳健的微孔板测定来确定棉花植株中诱导的 β-1,3-葡聚糖酶和几丁质酶活性。

由于病毒感染或外源性应用诱导物，棉花植株产生抗性。可以通过测定植物中 β-1,3-葡聚糖酶和几丁质酶的产量作为生化参数来评估诱导的抗性。用于测定几丁质酶和 β-1,3-葡聚糖酶活性的测定法既费力又不划算，因为底物胶体几丁质和海带多糖产生的还原糖非常昂贵。两种底物的浓度都被标准化，并在改良的微孔板测定中从 4% 降低到 0.25%，这似乎更有效。产生的 β-1,3-葡聚糖酶和几丁质酶的量很大，并由新的改良测定法确定。微孔板测定的灵敏度显着提高了大约一到两倍。