Control of glucose concentration has tremendous significance in medical diagnosis, pharmaceuticals, food, and fermentation industries. Herein, we report on the fabrication of a facile, low-cost, and sensitive enzyme-based amperometric sensor using the electrochemically deposited polyaniline (PANI) film on a graphite electrode. PANI was deposited from an aqueous solution of 0.2 M aniline in 1.0 M hydrocholoric acid (HCl) by cyclic voltammetry (CV). Surface morphology and composition characterization of the PANI film were carried out by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared (FTIR) spectroscopy. Potentiostatic immobilization of glucose oxidase (GO_X) enzyme in the PANI film was carried out at 0.75 V to fabricate an amperometric glucose biosensor (GOx/PANI/graphite biosensor). The glucose concentration response of the prepared sensor was studied amperometrically by detecting hydrogen peroxide (H₂O₂). The detection of H₂O₂ was optimized by calibrating the effects of pH, reduction potential, and background current. A reduction potential of -0.4 V at pH 6 was the best combination to get a maximum amperometric response of the GOx/PANI/graphite biosensor. A stable current response was obtained in 4 min with a high reproducibility in linearity within the concentration range of 0.01 M-0.1 M D-glucose. Therefore, the fabricated GOx/PANI/graphite biosensor could be a promising candidate for glucose sensing.

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聚苯胺涂层石墨上安培葡萄糖生物传感器的制备

葡萄糖浓度的控制在医学诊断，制药，食品和发酵工业中具有重要意义。在本文中，我们报告了在石墨电极上使用电化学沉积的聚苯胺（PANI）膜制造的一种便捷，低成本，敏感的基于酶的安培传感器。通过循环伏安法（CV）从0.2 M苯胺在1.0 M氢氯酸（HCl）中的水溶液中沉积PANI。通过扫描电子显微镜（SEM），X射线光电子能谱（XPS）和傅里叶变换红外（FTIR）光谱对PANI膜进行表面形貌和组成表征。葡萄糖氧化酶的恒电位固定化（GO _X在0.75V下进行PANI膜中的酶，以制造电流型葡萄糖生物传感器（GOx / PANI /石墨生物传感器）。通过检测过氧化氢（H ₂ O ₂），对安培传感器的葡萄糖浓度响应进行了安培分析。H ₂ O ₂的检测通过校准pH，还原电势和背景电流的影响优化pH。在pH 6时，-0.4 V的还原电位是获得GOx / PANI /石墨生物传感器最大安培响应的最佳组合。在0.01 M-0.1 M D-葡萄糖的浓度范围内，在4分钟内获得了稳定的电流响应，并且线性重复性很高。因此，制造的GOx / PANI /石墨生物传感器可能是葡萄糖传感的有前途的候选者。