Bordetella bronchiseptica (B. bronchiseptica) is an obligately aerobic, oxidase- and catalase-positive, nonfermentative Gram-negative coccobacillus. This study is aimed at examining the immune effects of Rg1, Rg1 plus oil, and other common adjuvants on inactivated B. bronchiseptica vaccine in rabbits. The mechanism underlying the adjuvant effect of Rg1 plus oil on the vaccine was also explored. Rg1 (100 μg) plus oil significantly improved the immune effect of B. bronchiseptica vaccine at both the humoral and cellular levels. Rg1-oil adjuvant increased the levels of IL-2 and IL-4 in rabbits after immunization. Rg1 (100 μg) plus oil also significantly increased TLR2 expression and downregulated NF-κB in splenocytes. Rg1-oil adjuvant may increase the levels of IL-2 and IL-4 via upregulating TLR2, thereby enhancing the immune effect of B. bronchiseptica vaccine. In conclusion, Rg1 plus oil could be used as a potential vaccine adjuvant for rabbit B. bronchiseptica vaccine.

中文翻译：

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Rg1-油佐剂诱导兔支气管败血博德特氏菌免疫反应的疗效

支气管炎博德特氏菌（乙。支气管炎）是一种专性需氧的，氧化酶和过氧化氢阳性，非发酵革兰氏阴性球杆菌。本研究旨在检验 Rg1、Rg1 加油和其他常见佐剂对灭活B的免疫作用。兔支气管败血病疫苗。还探讨了 Rg1 加油对疫苗的佐剂作用的潜在机制。Rg1 (100  μg ) 加油显着提高了B的免疫作用。体液和细胞水平的支气管败血病疫苗。Rg1-油佐剂增加免疫后兔体内IL-2和IL-4的水平。Rg1 (100  μ克）加油也显著增加TLR2表达和下调NF- κ在脾细胞B中。Rg1-油佐剂可能通过上调TLR2来增加IL-2和IL-4的水平，从而增强B的免疫作用。支气管败血症疫苗。总之，Rg1 加油可用作兔B的潜在疫苗佐剂。支气管败血症疫苗。