ABSTRACT

This study was to evaluate the effects of microwave device in melanin pigment staining procedures and to compare between the classical Masson-Fontana staining technique at room temperature and the Microwave technique with Masson-Fontana stain. A total of 50 skin tissue samples were taken for processing. After processing, two histochemical staining techniques were carried out; the classical Masson-Fontana method at room temperature and the microwave device Masson-Fontana staining method at temperature 25°C. Both methods were compared. This study indicated that the use of a microwave device in staining of melanin pigment reduced the time significantly (P ≤ 0.001) from overnight (24 hours) to only 2 minutes with excellent staining quality as compared to the classical Masson-Fontana method (P ≤ 0.001). In conclusion, the use of a microwave device is a vital tool for staining of melanin with the Masson-Fontana method as it is a time reducer and staining quality enhancer.

抽象的

这项研究旨在评估微波设备对黑色素色素染色程序的影响，并比较室温下的传统Masson-Fontana染色技术和Masson-Fontana染色的微波技术。总共采集了50个皮肤组织样本进行处理。加工后，进行了两种组织化学染色技术。在室温下使用经典的Masson-Fontana方法，在温度25°C下使用微波设备进行Masson-Fontana染色方法。比较了两种方法。这项研究表明，与经典的Masson-Fontana方法相比，使用微波设备对黑色素色素进行染色的时间从过夜（24小时）显着减少（P≤0.001）至仅2分钟，且染色质量极佳（P≤ 0.001）。综上所述，