当前位置：
X-MOL 学术
›
Ann. Appl. Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Multilocus sequence typing and phylogenetic analysis revealed two distinct almond witches'-broom phytoplasma subpopulations in Iran
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-01-28 , DOI: 10.1111/aab.12679 Niloofar Mosayyebi 1 , Zahra Mehraban 1 , Majid Siampour 1 , Ghobad Babaei 2 , Fabio Quaglino 3
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-01-28 , DOI: 10.1111/aab.12679 Niloofar Mosayyebi 1 , Zahra Mehraban 1 , Majid Siampour 1 , Ghobad Babaei 2 , Fabio Quaglino 3
Affiliation
|
During a field survey conducted in different geographical regions of Iran, phytoplasma-like symptoms were observed in peach, almond, wild almond and GF-677 rootstock. Based on 16S rDNA amplification, followed by nucleotide sequence and phylogenetic analyses, almond witches'-broom (AlmWB) phytoplasma strains were found in association with symptomatic plants. Based on the single-nucleotide polymorphisms within 16S rDNA sequence, most of the Iranian AlmWB phytoplasma strains were distinguished from the Lebanese strains. Further molecular typing, based on sequence and phylogenetic analyses of rplV-rpsC (ribosomal proteins) and imp (immunodominant membrane protein) genes, indicated that AlmWB phytoplasma strains can be differentiated in two subpopulations: Ir, including exclusively Iranian strains; Lb, comprising strains from Lebanon and Iran. Moreover, the selection pressure analysis of imp gene sequences suggested that these two AlmWB phytoplasma subpopulations had some distinct biological or ecological properties. Finally, results of the sequence and phylogenetic analyses performed on rplV-rpsC and imp genes showed a considerably high genetic distance between AlmWB phytoplasma strains (16SrIX-B and variants) and phytoplasmas belonging to other 16SrIX subgroups. The significance of genetic variation in phytoplasmas of 16SrIX group is discussed in relation to their biological characteristics and geographical distinction.
中文翻译：
多位点序列分型和系统发育分析揭示了伊朗两个不同的杏仁女巫-扫帚植原体亚群
在伊朗不同地理区域进行的实地调查中，在桃、杏仁、野生杏仁和 GF-677 砧木中观察到类似植原体的症状。基于 16S rDNA 扩增，随后进行核苷酸序列和系统发育分析，发现了与有症状植物相关的杏仁女巫 (AlmWB) 植原体菌株。基于 16S rDNA 序列内的单核苷酸多态性，大多数伊朗 AlmWB 植原体菌株与黎巴嫩菌株区分开来。进一步的分子分型，基于rplV-rpsC（核糖体蛋白）和imp 的序列和系统发育分析（免疫显性膜蛋白）基因，表明 AlmWB 植原体菌株可以区分为两个亚群：Ir，仅包括伊朗菌株；Lb，包括来自黎巴嫩和伊朗的菌株。此外，imp基因序列的选择压力分析表明，这两个 AlmWB 植原体亚群具有一些不同的生物学或生态学特性。最后，对rplV-rpsC和imp进行的序列和系统发育分析的结果基因显示 AlmWB 植原体菌株（16SrIX-B 和变体）与属于其他 16SrIX 亚群的植原体之间的遗传距离相当高。讨论了 16SrIX 组植原体遗传变异的重要性，并结合它们的生物学特性和地理特征进行了讨论。
更新日期：2021-01-28
中文翻译：
多位点序列分型和系统发育分析揭示了伊朗两个不同的杏仁女巫-扫帚植原体亚群
在伊朗不同地理区域进行的实地调查中，在桃、杏仁、野生杏仁和 GF-677 砧木中观察到类似植原体的症状。基于 16S rDNA 扩增，随后进行核苷酸序列和系统发育分析，发现了与有症状植物相关的杏仁女巫 (AlmWB) 植原体菌株。基于 16S rDNA 序列内的单核苷酸多态性，大多数伊朗 AlmWB 植原体菌株与黎巴嫩菌株区分开来。进一步的分子分型，基于rplV-rpsC（核糖体蛋白）和imp 的序列和系统发育分析（免疫显性膜蛋白）基因，表明 AlmWB 植原体菌株可以区分为两个亚群：Ir，仅包括伊朗菌株；Lb，包括来自黎巴嫩和伊朗的菌株。此外，imp基因序列的选择压力分析表明，这两个 AlmWB 植原体亚群具有一些不同的生物学或生态学特性。最后，对rplV-rpsC和imp进行的序列和系统发育分析的结果基因显示 AlmWB 植原体菌株（16SrIX-B 和变体）与属于其他 16SrIX 亚群的植原体之间的遗传距离相当高。讨论了 16SrIX 组植原体遗传变异的重要性，并结合它们的生物学特性和地理特征进行了讨论。
京公网安备 11010802027423号