Derivatization reactions are commonly used in mass spectrometry to improve analyte signals, specifically by enhancing the ionization efficiency of those compounds. Vicinal diols are one group of biologically important compounds that have been commonly derivatized using boronic acid. In this study, a boronic acid with a tertiary amine was adapted for the derivatization of vicinal diol metabolites in B73 maize tissue cross‐sections for mass spectrometry imaging analysis. Using this method, dozens of vicinal diol metabolites were derivatized, effectively improving the signal of those metabolites. Many of these metabolites were tentatively assigned using high‐resolution accurate mass measurements. In addition, reaction interference and cross‐reactivity with various other functional groups were systematically studied to verify data interpretation.

中文翻译：

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组织上硼酸衍生化用于通过 MALDI-MS 成像分析玉米中的邻二醇代谢物

衍生化反应通常用于质谱分析以改善分析物信号，特别是通过提高这些化合物的电离效率。邻二醇是一组具有重要生物学意义的化合物，通常使用硼酸进行衍生化。在这项研究中，一种带有叔胺的硼酸适用于 B73 玉米组织横截面中邻二醇代谢物的衍生化，用于质谱成像分析。使用该方法衍生了数十种邻位二醇代谢物，有效改善了这些代谢物的信号。许多这些代谢物是使用高分辨率精确质量测量暂时分配的。此外，系统地研究了与各种其他官能团的反应干扰和交叉反应性，以验证数据解释。