Vitamin D plays an important role in many physiological processes, particularly calcium and phosphorous homeostasis. The biochemistry of vitamin D is also complex, encompassing a range of active molecules that may be either endogenous or dietary in origin. The role of lipids and fats in the production, processing and use of vitamin D is an interesting one, with a relative paucity of model studies into the interactions of vitamin D with lipidic systems such as micelles and vesicles. Here, we have studied the effect of vitamin D3 in simple unsaturated phospholipid systems. We used NMR and FTIR spectroscopy to investigate the effect of increasing vitamin D concentration on the structure and dynamics of the lipid chains and interfacial region. In order to link these model studies with more complex biomimetic environments, we compare results in the presence of buffer and vitamin D binding protein. We have also used DLS to determine that vitamin D3-DOPC vesicles can retain their size distribution for varying amounts of time in different conditions. We find that the acyl chain region of vitamin D3-DOPC membranes are generally disordered, and that the addition of buffer and/or protein alters the properties of the interfacial region.

维生素 D 在许多生理过程中起着重要作用，尤其是钙和磷的稳态。维生素 D 的生物化学也很复杂，包含一系列活性分子，这些分子可能是内源性的，也可能是饮食来源的。脂质和脂肪在维生素 D 的生产、加工和使用中的作用是一个有趣的问题，相对缺乏对维生素 D 与脂质系统（如胶束和囊泡）相互作用的模型研究。在这里，我们研究了维生素 D3 在简单不饱和磷脂系统中的作用。我们使用 NMR 和 FTIR 光谱来研究增加维生素 D 浓度对脂质链和界面区域的结构和动力学的影响。为了将这些模型研究与更复杂的仿生环境联系起来，我们比较了存在缓冲液和维生素 D 结合蛋白时的结果。我们还使用 DLS 来确定维生素 D3-DOPC 囊泡可以在不同条件下在不同时间段内保持其大小分布。我们发现维生素 D3-DOPC 膜的酰基链区域通常是无序的，并且添加缓冲液和/或蛋白质会改变界面区域的特性。