Micro-fluidized bed (MFB) can be used as a fast screening tool for preliminary testing of engineering designs, but the applications of MFB for bioprocessing research such as microorganism cultivation is still deficient. This paper reports the unique fluidization performance of fungal pellets in a 3D-printed micro-fluidized bed, thus giving insights to the hydrodynamic study of pellets and broadening the potential of MFB in the bioprocessing and bioproduction fields. The results indicated that the static bed voidage were substantially larger (around 0.6) than the conventionally used value of 0.4. The observed decrease in packed bed voidage with increasing pellet mass was attributed to the increased compaction of pellets. A novel extended bed regime was observed prior to partially and fully fluidized bed regimes for liquid-solid fluidization. Due to the wall effect and surface forces, higher values of the minimum fluidization velocity and Richardson-Zaki empirical constants (n and K) were determined. In liquid-solid-gas fluidization system, fluidization regimes including packed-bed regime, fluidization regime and elutriation regimes were mapped. The effects on gas flow and pellet weight on fluidization performance were also investigated. It was noticed that pellets were fully fluidized at low biomass weight (i.e. 0.5 g) but the increase of pellet mass led to partially fluidization where the packed bed height increased with increasing pellet mass but decreased with increasing gas flowrate. Pellet fluidization velocity including axial velocity and radial velocity were measured by PIVlab analysis. The results indicated that pellet axial velocity was mainly affected by vertical gas flow while radial velocity was dominated by vortexes and surface forces.

微流化床（MFB）可以用作对工程设计进行初步测试的快速筛选工具，但是MFB在生物加工研究（例如微生物培养）中的应用仍然缺乏。本文报道了在3D打印的微流化床中真菌颗粒的独特流化性能，从而为颗粒的流体动力学研究提供了见识，并拓宽了MFB在生物加工和生物生产领域的潜力。结果表明，静态床空隙率比常规使用的值0.4大得多（约0.6）。随着粒料质量的增加，填充床空隙的减少归因于粒料的致密性增加。在部分和完全流化床方案进行液-固流化之前，观察到一种新颖的延长床方案。确定n和K）。在液-固-气流化系统中，绘制了包括填充床流态，流化态和淘析态的流态化图。还研究了气流和颗粒重量对流化性能的影响。注意到颗粒在低生物量（即0.5g）下完全流化，但是颗粒质量的增加导致部分流化，其中填充床高度随着颗粒质量的增加而增加，但随着气体流量的增加而降低。通过PIVlab分析测量颗粒流化速度，包括轴向速度和径向速度。结果表明，颗粒的轴向速度主要受垂直气流的影响，而径向速度则受旋涡和表面力的支配。