Intensive greenhouse vegetable production is one of the most important economic activities in south-east Spain. Agricultural intensification has limited the application of organic matter (OM) in favor of synthetic chemical fertilizers, leading to altered soil microbial communities and production loss. We addressed the effects of soil microbial communities on plant productivity depending on different soil management practices in greenhouse agriculture. Greenhouse managements were i) conventional (i.e., with addition of synthetic chemical fertilizers) without organic amendments (CM), ii) conventional with organic amendments (CMOM) and with addition of synthetic chemical fertilizers, and iii) organic management with annual organic amendments and with no synthetic chemical fertilizer addition (ORG). We extracted soil microbial communities from five greenhouses per management type, added these inocula to pots with sterile substrate (peat:sand), and seeded disinfected tomato seeds. Plants grew for 2 months without nutrient addition (only water irrigation). Immediately prior to the end of the study, we measured photosynthetic rate, plant growth, and leaf functional traits. At the end of study, pot substrates inoculated with i) ORG soil communities had higher bacterial abundance (qPCR) compared to microbial communities inoculated with CM extracts; and ii) prokaryotic communities of CM and CMOM substrates differed in composition. Besides, substrates inoculated with CM inocula were significantly associated with the presence of a potential fungal pathogen. Plants inoculated with extracts from organic greenhouses grew more and had a higher photosynthetic rate than plants receiving soil microbial extracts from greenhouses with conventional management. There were differences in the NO₃⁻, total N and organic matter contents of substrates between treatments at the end of the study, but not at the beginning of the study, suggesting differences in microbial activity between treatments. Although substrate N content differences did not translate into differences in leaf N, leaf δ¹⁵N differed between treatments, indicating that the source of the soil microbial inocula influenced the N compound used by the tomato plants. Overall, this study suggested that soil microbial communities from organic greenhouses had a positive effect on crop productivity in intensive greenhouse agriculture.

集约化温室蔬菜生产是西班牙东南部最重要的经济活动之一。农业集约化已经限制了有机物（OM）的应用，而倾向于合成化学肥料，导致土壤微生物群落的改变和生产损失。我们根据温室农业中不同的土壤管理实践，探讨了土壤微生物群落对植物生产力的影响。温室管理是：i）常规的（即添加合成化肥）而没有有机改良剂（CM），ii）常规的有机改良剂（CMOM）和合成化肥，以及iii）年度有机修正的有机管理和无需添加合成化学肥料（ORG）。我们从每种管理类型的五个温室中提取了土壤微生物群落，并将这些接种物添加到带有无菌基质（豌豆：沙）的盆中，并播种了消毒的番茄种子。植物生长了两个月，没有添加任何营养（仅水灌溉）。在研究即将结束之前，我们测量了光合速率，植物生长和叶片功能性状。在研究结束时，接种i）ORG土壤群落的盆栽基质比接种CM提取物的微生物群落具有更高的细菌丰度（qPCR）；ii）CM和CMOM底物的原核群落组成不同。此外，接种CM接种物的底物与潜在的真菌病原体的存在显着相关。接种有机大棚提取物的植物比接受常规管理的大棚土壤微生物提取物的植物生长更快，光合速率更高。NO有所不同₃ ^- ，全N，并在研究的结束时处理之间基材的有机物含量，而不是在研究开始时，提示治疗之间在微生物活性的差异。虽然衬底N含量的差异并没有转化为在叶片N的差异，叶δ ¹⁵ Ñ不同处理之间，表明土壤微生物菌剂的源的影响所使用的番茄植物的N化合物。总体而言，这项研究表明，有机温室中的土壤微生物群落对集约型温室农业中的作物生产力具有积极影响。