Curtailing the absorption of triglycerides (TGs) is a preferred pathway for treating obesity. Our previous study demonstrated that the water-soluble fraction from potato could inhibit the lipase activity of patatin, one of the major proteins in potato. This aqueous fraction was purified and concentrated by deproteination and reversed-phase chromatography to investigate the effectiveness against obesity. Biochemical analyses indicated that the fraction non-competitively inhibited pancreatic lipase (PLase) with a half-maximal inhibitory concentration of 10.17 µg/mL, and was named as potato-derived lipase inhibitory fraction (PI). Animal studies on C57BL/6 mice showed that in mice fed a high-fat diet (HFD), PI treatment resulted in reductions in body weight gain, adipose fat deposition, and liver TGs, and ameliorated the gut microbiome dysbiosis caused by HFD feeding; meanwhile, orlistat, a well-known lipase inhibitor, diverged the gut microbiome profile in mice fed a HFD. High resolution electronspray ionization-Orbitrap tandem mass spectrometry identified gallic acid, 4-hydroxybenzoic acid, and protocatechuic acid, which are known to have lipase inhibitory activities, in PI. However, these compounds could not reconstitute comparable specific inhibitory activity of PI inferring the existence of another inhibitory compound(s) to be identified in PI.

中文翻译：

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马铃薯提取物抑制高脂饮食小鼠的脂肪酶活性并改善肠道微生物组营养不良和体重增加

减少甘油三酸酯（TGs）的吸收是治疗肥胖症的首选途径。我们以前的研究表明，马铃薯中的水溶性部分可以抑制马铃薯抑肽酶（马铃薯中的主要蛋白质之一）的脂肪酶活性。通过脱蛋白和反相色谱法纯化和浓缩该水相部分，以研究抗肥胖的功效。生化分析表明，该组分非竞争性抑制胰脂肪酶（PLase）的半数最大抑制浓度为10.17 µg / mL，被称为马铃薯来源的脂肪酶抑制组分（PI）。对C57BL / 6小鼠的动物研究表明，在高脂饮食（HFD）喂养的小鼠中，PI处理可降低体重增加，脂肪沉积和肝脏TG，并改善了HFD喂养引起的肠道微生物组营养不良；同时，众所周知的脂肪酶抑制剂奥利司他（Orlistat）使喂食HFD的小鼠的肠道微生物组谱发生了变化。高分辨率电子喷雾电离-Orbitrap串联质谱分析法确定了PI中的没食子酸，4-羟基苯甲酸和原儿茶酸，它们已知具有脂肪酶抑制活性。但是，这些化合物不能重构PI的可比特异性抑制活性，从而推断出在PI中还存在另一种抑制化合物。在PI中已知具有脂肪酶抑制活性。但是，这些化合物不能重构PI的可比特异性抑制活性，从而推断出在PI中还存在另一种抑制化合物。在PI中已知具有脂肪酶抑制活性。但是，这些化合物不能重构PI的可比特异性抑制活性，从而推断出在PI中还存在另一种抑制化合物。