In this article the total reflection X-ray fluorescence (TXRF) analysis was used for determination of element concentrations in three isolated peripheral blood components: lymphocytes, erythrocytes, and plasma, collected from 36 healthy donors (15 men and 21 women) from eastern Poland. The studied blood components were isolated from whole peripheral blood using Histopaque-1077 density gradient centrifugation. In the lymphocytes, the following elements were measured: P, S, Cl, K, Ca, Fe, Zn, Br, Sr and Pb. In the erythrocytes: P, S, Cl, K, Cr, Mn, Fe, Cu, Zn, Se, Br, Rb and Pb, while in the plasma samples: P, S, Cl, K, Ti, V, Cr, Fe, Co, Ni, Cu, Zn, Br, Se, Sr and Pb. The descriptive statistic parameters of concentration such as: mean value, standard deviation, median, 1^st and 3^rd quartiles, 10^th and 90^th percentiles were calculated for all samples of the studied blood components, as well as separately for male and female groups. The measured element concentrations and calculated parameters can be used as the reference values. Element concentration distributions for male and female groups were statistically compared using the non-parametric Mann-Whitney test and statistical significance differences (α = 0.05) were found for: P (in lymphocytes), Se and Rb (in erythrocytes) and V (in plasma). The multi-group statistical comparison of element concentration distribution for different blood components was also done (Mann-Whitney and Kruskal-Wallis tests). The statistical test show that, the concentration levels are usually different, except in the following cases: Zn, Fe (lymphocytes and plasma, Fe only for female group), Cr (erythrocytes and plasma). The obtained concentration ranges were compared with literature-based data available for element concentration in lymphocytes, erythrocytes, and plasma. In this work, the application of the TXRF method allowed additionally for the simultaneous determination of the concentrations of such elements as: P, S, Cl, Br, Sr and Pb in lymphocytes, S, Cl and Br in erythrocytes, and P, S, Cl, K, Ti, Br and Pb in plasma.

From analytical point of view, the article describes in details the measurement procedure including the isolation of blood components and samples preparation for TXRF measurements, and later the detection limit of the method is discussed.

在这篇文章中，全反射 X 射线荧光 (TXRF) 分析用于测定三种分离的外周血成分中的元素浓度：淋巴细胞、红细胞和血浆，这些成分来自波兰东部的 36 名健康捐献者（15 名男性和 21 名女性） . 使用 Histopaque-1077 密度梯度离心从全外周血中分离研究的血液成分。在淋巴细胞中，测量了以下元素：P、S、Cl、K、Ca、Fe、Zn、Br、Sr 和 Pb。在红细胞中：P、S、Cl、K、Cr、Mn、Fe、Cu、Zn、Se、Br、Rb 和 Pb，而在血浆样品中：P、S、Cl、K、Ti、V、Cr、铁、钴、镍、铜、锌、溴、硒、锶和铅。浓度的描述性统计参数如：平均值、标准差、中位数、第1^st和第3^rd四分位数、10< sup>th</sup> 和 90^th 个百分位数被计算为所有研究的血液成分样本，以及分别为男性和女性组计算。测量的元素浓度和计算的参数可用作参考值。使用非参数 Mann-Whitney 检验对男性和女性组的元素浓度分布进行了统计比较，发现了以下各项的统计显着性差异 (α = 0.05)：P（在淋巴细胞中）、Se 和 Rb（在红细胞中）和 V（在血浆中）。还对不同血液成分的元素浓度分布进行了多组统计比较（Mann-Whitney 和 Kruskal-Wallis 检验）。统计检验表明，浓度水平通常不同，但以下情况除外：Zn、Fe（淋巴细胞和血浆、Fe 仅用于女性组），Cr（红细胞和血浆）。将获得的浓度范围与有关淋巴细胞、红细胞和血浆中元素浓度的文献数据进行比较。在这项工作中，TXRF 方法的应用还允许同时测定以下元素的浓度：淋巴细胞中的 P、S、Cl、Br、Sr 和 Pb，红细胞中的 S、Cl 和 Br，以及 P、S 、Cl、K、Ti、Br 和 Pb 等离子。

从分析的角度，文章详细描述了测量程序，包括血液成分的分离和 TXRF 测量的样品制备，然后讨论了该方法的检测限。