Alphavirus M1 is a promising oncolytic virus for cancer therapy. Here, we constructed a fluorescent reporter virus for real-time visualization and quantification of M1 virus both in vitro and in vivo. The reporter-encoding M1 virus maintained the characteristics of parental virus in the aspects of structure, replication capacity, the feature to induce cytopathic cell death, and the property of tumor targeting. The fluorescence is positively correlated with virus replication both in vitro and in vivo. More importantly, the reporter can be stably expressed for at least 10 generations in a serial passage assay. In summary, we successfully constructed stable and authentic reporter viruses for studying M1 virus and provided a feasible technical route for gene modification of oncolytic virus M1.

中文翻译：

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体外和体内溶瘤 M1 病毒的实时可视化和量化

甲病毒 M1 是一种用于癌症治疗的有前途的溶瘤病毒。在这里，我们构建了一种荧光报告病毒，用于在体外和体内对 M1 病毒进行实时可视化和量化。编码报告基因的M1病毒在结构、复制能力、诱导细胞病变性细胞死亡的特征和肿瘤靶向性等方面保持了亲本病毒的特征。荧光与体外和体内病毒复制呈正相关. 更重要的是，报告基因可以在连续传代试验中稳定表达至少 10 代。综上所述，我们成功构建了用于研究M1病毒的稳定可靠的报告病毒，为溶瘤病毒M1的基因修饰提供了一条可行的技术路线。