Salinity and drought are major abiotic stresses affecting wheat (Triticum aestivum L.) production throughout the world, and discovery of loci for traits affecting yield under salinity may lead to the breeding for salt-tolerant plants. In the present study, 186 F₁₀ recombinant inbred line (RIL) populations were evaluated under salt-stress conditions in order to identify main-effect and epistatic-effect quantitative trait loci (QTLs) for 15 traits in wheat during the germination and early-seedling stages. In total, 61 main-effect QTLs on 15 chromosomes and 21 epistatic interactions on 12 chromosomes were detected through composite interval mapping (CIM) and a mixed-model-based CIM method. Two major QTLs for primary-leaf fresh weight and coleoptile fresh weight were detected on chromosome (or linkage group) 5B₂ and 2D, respectively, which contributed ~44% and 43% of the phenotypic variance. Additionally, 12 QTL clusters including different traits were detected on 1A₁, 3A, 4A, 2B₁, 3B, 5B₁ and 2D₁. Candidate genes were identified within QTL regions and gene ontology (GO) enrichment analysis was performed. In total, 9134 candidate genes were grouped into 274 GO terms (including 79 GO terms involved in the ‘biological process’ category). These genes directly or indirectly play a vital role such as lipid localisation, biological regulation, fatty acid biosynthetic process, cellular process, DNA conformation change, translational elongation, carbohydrate metabolic process, Fe ion homeostasis, hydrogen peroxide metabolic process, and pigment biosynthetic process at the germination and early-seedling stages under salt-stress conditions.

盐度和干旱是影响世界各地小麦（Triticum aestivum L.）产量的主要非生物胁迫，而在盐度下发现影响产量的性状的基因座可能会导致耐盐植物的育种。在本研究中，186 F ₁₀在盐胁迫条件下对重组自交系（RIL）群体进行了评估，以鉴定萌发和早期苗期小麦中15个性状的主要效应和上位效应定量性状位点（QTL）。通过复合间隔图（CIM）和基于混合模型的CIM方法，共检测到15个染色体上的61个主要效应QTL和12个染色体上的21个上位相互作用。在染色体（或连锁群）5B ₂和2D上分别检测到两个主要的初生叶鲜重和胚芽鞘鲜重的QTL ，它们贡献了约44％和43％的表型变异。此外，在图1A中检测到12个QTL簇，包括不同性状₁，3A，4A，2B ₁，图3B，图5B ₁和2D₁。在QTL区域内鉴定候选基因，并进行基因本体论（GO）富集分析。总共将9134个候选基因分为274个GO术语（包括“生物过程”类别中涉及的79个GO术语）。这些基因直接或间接地起着至关重要的作用，例如脂质定位，生物调节，脂肪酸生物合成过程，细胞过程，DNA构象变化，翻译延伸，碳水化合物代谢过程，Fe离子稳态，过氧化氢代谢过程和色素生物合成过程。盐胁迫条件下的发芽和早苗阶段。