Carbapenem-resistant Acinetobacter spp. are considered priority drug-resistant human-pathogenic bacteria. The genomes of two carbapenem-resistant Acinetobacter spp. clinical isolates obtained from the same tertiary hospital in Terengganu, Malaysia, namely, A. baumannii AC1633 and A. nosocomialis AC1530, were sequenced. Both isolates were found to harbor the carbapenemase genes bla_NDM-1 and bla_OXA-58 in a large (ca. 170 kb) plasmid designated pAC1633-1 and pAC1530, respectively, that also encodes genes that confer resistance to aminoglycosides, sulfonamides, and macrolides. The two plasmids were almost identical except for the insertion of ISAba11 and an IS4 family element in pAC1633-1, and ISAba11 along with relBE toxin-antitoxin genes flanked by inversely orientated pdif (XerC/XerD) recombination sites in pAC1530. The bla_NDM-1 gene was encoded in a Tn125 composite transposon structure flanked by ISAba125, whereas bla_OXA-58 was flanked by ISAba11 and ISAba3 downstream and a partial ISAba3 element upstream within a pdif module. The presence of conjugative genes in plasmids pAC1633-1/pAC1530 and their discovery in two distinct species of Acinetobacter from the same hospital are suggestive of conjugative transfer, but mating experiments failed to demonstrate transmissibility under standard laboratory conditions. Comparative sequence analysis strongly inferred that pAC1633-1/pAC1530 was derived from two separate plasmids in an IS1006-mediated recombination or transposition event. A. baumannii AC1633 also harbored three other plasmids designated pAC1633-2, pAC1633-3, and pAC1633-4. Both pAC1633-3 and pAC1633-4 are cryptic plasmids, whereas pAC1633-2 is a 12,651-bp plasmid of the GR8/GR23 Rep3-superfamily group that encodes the tetA(39) tetracycline resistance determinant in a pdif module.

中文翻译：

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鲍曼不动杆菌 AC1633 和院内不动杆菌 AC1530 的完整基因组测序揭示了编码 NDM-1 和 OXA-58 碳青霉烯酶的大型多重耐药质粒


耐碳青霉烯类不动杆菌属被认为是优先耐药的人类致病菌。两种耐碳青霉烯类不动杆菌属的基因组。对从马来西亚登嘉楼州同一家三级医院获得的临床分离株鲍曼不动杆菌AC1633 和院内不动杆菌AC1530 进行了测序。发现这两种分离株在分别命名为 pAC1633-1 和 pAC1530 的大（约 170 kb）质粒中含有碳青霉烯酶基因bla _NDM-1和bla _OXA-58 ，该质粒还编码赋予对氨基糖苷类、磺胺类和磺胺类药物抗性的基因。大环内酯类。除了在 pAC1633-1 中插入 IS Aba11和 IS 4家族元件之外，以及在 pAC1530 中插入 IS Aba11和relBE毒素-抗毒素基因（侧翼为反向 p diff (XerC/XerD) 重组位点）之外，这两个质粒几乎相同。 bla _NDM-1基因在侧翼为 IS Aba125的 Tn 125复合转座子结构中编码，而bla _OXA-58侧翼为 IS Aba11和 IS Aba3下游以及 ap dif模块内的部分 IS Aba3元件上游。质粒 pAC1633-1/pAC1530 中存在接合基因，并且在同一家医院的两种不同不动杆菌属中发现接合基因，这表明存在接合转移，但交配实验未能证明标准实验室条件下的传播性。 比较序列分析强烈推断pAC1633-1/pAC1530源自IS 1006介导的重组或转座事件中的两个单独的质粒。鲍曼不动杆菌AC1633 还含有另外三个质粒，分别命名为 pAC1633-2、pAC1633-3 和 pAC1633-4。 pAC1633-3 和 pAC1633-4 都是隐性质粒，而 pAC1633-2 是 GR8/GR23 Rep3 超家族组的 12,651 bp 质粒，编码 ap dif模块中的tetA ( 39 ) 四环素抗性决定簇。