In this article, it intends to find out the dominant strains on the anode biofilm that prominently contribute to the improvement of biogas quality and methane (CH₄) conversion, the relationships between the gas-producing potential, electricity-generating capacity, and anode biofilm microbial characteristics were investigated by using volatile fatty acids (intermediates of AD) and glucose as substrates. The experimental results showed that the gas-producing potential and electricity-generating capacity of EAD systems both are acetate > butyrate > propionate > glucose. The acetate experimental group had better gas-producing potential and higher content of hydrogen and methane accounting for 16.12 and 82.61%, respectively. The electrochemical activity of anodic biofilms has been proved to be positively correlated with the electricity-generating capacity of different substrates, and the acetate experimental group had a greater electricity-generating capacity with a peak current of 23.07 mA and a coulombic efficiency of 57.69%. The more remarkable gas-producing potential and electricity-generating capacity of the experimental group are, and the larger quantities of electroactive microorganisms on the anode biofilm is, there are two strains commonly shared by four different substrate anode biofilms that contribute the most to improve biogas quality and CH₄ yield, namely F. Clostridium Perfringens with a hydrogen-producing ability and P. Geobacter Lovleyi with a power-generating capacity. All results concerned above will contribute to enormously improve the CH₄ conversion rate and the biogas quality. Moreover, these electroactive microorganisms also have very broad application prospects in terms of organic sewage treatment and kitchen waste treatment.

中文翻译：

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基于不同底物电化学厌氧消化的产气、发电和阳极生物膜微生物特性

在本文中，它旨在找出阳极生物膜上对改善沼气质量和甲烷（CH ₄) 转化，通过使用挥发性脂肪酸（AD 的中间体）和葡萄糖作为底物，研究了产气潜力、发电能力和阳极生物膜微生物特性之间的关系。实验结果表明，EAD系统的产气潜力和发电能力均为乙酸>丁酸>丙酸>葡萄糖。醋酸盐实验组产气潜力较好，氢气和甲烷含量分别为16.12%和82.61%。阳极生物膜的电化学活性已被证明与不同基材的发电能力呈正相关，醋酸实验组的发电能力更大，峰值电流为23。07 mA 和 57.69% 的库仑效率。实验组产气潜力和发电能力越显着，阳极生物膜上电活性微生物的数量越多，四种不同基质阳极生物膜共有的两种菌株对改善沼气的贡献最大。质量和CH₄产量，即具有产氢能力的产气荚膜梭菌和具有发电能力的P. Geobacter Lovleyi。上述所有相关结果都将有助于极大地提高 CH ₄转化率和沼气质量。而且，这些电活性微生物在有机污水处理和餐厨垃圾处理方面也有着非常广阔的应用前景。