Use of continuous sample translation to reduce radiation damage for XPCS studies of protein diffusion,Journal of Synchrotron Radiation

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Use of continuous sample translation to reduce radiation damage for XPCS studies of protein diffusion
Journal of Synchrotron Radiation ( IF 2.4 ) Pub Date : 2021-01-28 , DOI: 10.1107/s1600577521000035
Laurence B Lurio ₁ , George M Thurston ₂ , Qingteng Zhang ₃ , Suresh Narayanan ₃ , Eric M Dufresne ₃

Affiliation

An experimental setup to measure X‐ray photon correlation spectroscopy during continuous sample translation is presented and its effectiveness as a means to avoid sample damage in dynamics studies of protein diffusion is evaluated. X‐ray damage from focused coherent synchrotron radiation remains below tolerable levels as long as the sample is translated through the beam sufficiently quickly. Here it is shown that it is possible to separate sample dynamics from the effects associated with the transit of the sample through the beam. By varying the sample translation rate, the damage threshold level, D_thresh = 1.8 kGy, for when beam damage begins to modify the dynamics under the conditions used, is also determined. Signal‐to‐noise ratios, R_sn ≥ 20, are obtained down to the shortest delay times of 20 µs. The applicability of this method of data collection to the next generation of multi‐bend achromat synchrotron sources is discussed and it is shown that sub‐microsecond dynamics should be obtainable on protein samples.

中文翻译：

使用连续样品平移来减少蛋白质扩散 XPCS 研究的辐射损伤

提出了一种在连续样品平移过程中测量 X 射线光子相关光谱的实验装置，并评估了其作为避免蛋白质扩散动力学研究中样品损坏的手段的有效性。只要样品足够快地通过光束平移，聚焦相干同步加速器辐射造成的 X 射线损伤仍低于可容忍水平。此处表明，可以将样品动力学与与样品通过光束的传输相关联的影响分开。通过改变样本平移率，还可以确定损伤阈值水平D _thresh = 1.8 kGy，因为当光束损伤开始改变所使用条件下的动力学时。信噪比，R _sn≥ 20，最短延迟时间为 20 µs。讨论了这种数据收集方法对下一代多弯曲消色差同步加速器源的适用性，并表明亚微秒动力学应该可以在蛋白质样品上获得。

更新日期：2021-03-04

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