Reversible crosslinked assembly of a trimeric coiled-coil peptide into a three-dimensional matrix for cell encapsulation and release,Journal of Peptide Science

当前位置： X-MOL 学术 › J. Pept. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Reversible crosslinked assembly of a trimeric coiled-coil peptide into a three-dimensional matrix for cell encapsulation and release
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2021-01-27 , DOI: 10.1002/psc.3302
Michael D Jorgensen ₁ , Jean Chmielewski ₁

Affiliation

Mimicking the extracellular matrix (ECM) continues to be a goal in the field of regenerative medicine. Herein, we report a modified trimeric GCN4 coiled-coil sequence containing three ligands for metal ions specifically positioned for crosslinked assembly (TriCross). In the presence of metal ions, TriCross assembles into a three-dimensional (3D) matrix with significant cavities to accommodate cells. The matrix was found to be stable in media with serum, and mild removal of the metal leads to disassembly. By assembling TriCross with a suspension of cells in media, the matrix encapsulates cells during the assembly process leading to high cell viability. Further disassembly under mild conditions allows for the release of cells from the scaffold. As such, this peptide-based material displays many of the characteristics necessary for successful 3D cell culture.

中文翻译：

三聚体卷曲螺旋肽的可逆交联组装成用于细胞封装和释放的三维矩阵

模仿细胞外基质 (ECM) 仍然是再生医学领域的目标。在这里，我们报告了一种修饰的三聚体 GCN4 卷曲螺旋序列，其中包含三个金属离子配体，专门用于交联组装 ( TriCross )。在金属离子存在的情况下，TriCross组装成具有大量空腔以容纳细胞的三维 (3D) 矩阵。发现该基质在含有血清的介质中是稳定的，金属的轻微去除会导致分解。通过组装TriCross通过在培养基中悬浮细胞，基质在组装过程中封装细胞，从而提高细胞活力。在温和条件下进一步拆卸允许细胞从支架上释放。因此，这种基于肽的材料显示出成功的 3D 细胞培养所必需的许多特征。

更新日期：2021-01-27

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11