Degradation of Erythromycin by a Novel Fungus, Penicillium oxalicum RJJ-2, and the Degradation Pathway,Waste and Biomass Valorization

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Degradation of Erythromycin by a Novel Fungus, Penicillium oxalicum RJJ-2, and the Degradation Pathway
Waste and Biomass Valorization ( IF 2.6 ) Pub Date : 2021-01-27 , DOI: 10.1007/s12649-021-01343-y
Jianjun Ren , Zhenzhu Wang , Liujie Deng , Dongze Niu , Huhetaoli , Zhijie Li , Liping Dong , Jin Zhang , Ruitao Zhang , Chunyu Li

Purpose

This study aimed to isolate effective erythromycin-degrading fungi and determine the characteristics and pathway of degradation.

Methods

Erythromycin-degrading fungi were isolated from erythromycin-contaminated samples using a standard enrichment and isolation method. The degradation characteristics were investigated in mineral salt medium (MSM) with erythromycin as a sole carbon source. Key degradation intermediates were analyzed by high performance liquid chromatography–mass spectrometry (HPLC–MS) and used to deduce the erythromycin degradation pathway of strain RJJ-2.

Results

A novel erythromycin-degrading fungus RJJ-2, was isolated from a contaminated sample. Based on its morphology and internal transcribed spacer (ITS) sequence, the strain was 100% similar to P. oxalicum (MN759650) and named P. oxalicum RJJ-2. The strain RJJ-2 degraded 84.88% erythromycin after 96-h incubation at 35 °C and pH 6.0 in MSM with erythromycin (100 mg L⁻¹) as the sole carbon source. Optimal degradation conditions for P. oxalicum RJJ-2 were 35 °C, and pH 6.0 with 0.1% ammonium sulfate supplementation. HPLC–MS analysis indicated that the main degradation intermediates were 3-depyranosyloxy erythromycin A, cladinose, desosamine, and 7,12-dyhydroxy-6-deoxyerythronolide B. It was inferred that the erythromycin was degraded to 3-depyranosyloxy erythromycin A by a glycoside hydrolase in the initial reaction.

Conclusion

This study demonstrated that P. oxalicum RJJ-2 is a novel erythromycin-degrading strain, which can provide a new eco-friendly and cost-effective approach for the disposal of erythromycin fermentation wastes and other hazardous chemicals.

Graphic Abstract

中文翻译：

新型真菌草酸青霉RJJ-2对红霉素的降解及其降解途径

目的

这项研究旨在分离有效的红霉素降解真菌，并确定其特征和降解途径。

方法

使用标准富集和分离方法，从被红霉素污染的样品中分离出降解红霉素的真菌。在以红霉素为唯一碳源的矿物盐培养基（MSM）中研究了降解特性。通过高效液相色谱-质谱（HPLC-MS）分析了关键的降解中间体，并将其推导到RJJ-2菌株的红霉素降解途径。

结果

从受污染的样品中分离出了一种新型的红霉素降解真菌RJJ-2。基于其形态和内部转录间隔区（ITS）序列，该菌株与草假单胞菌（MN759650）100％相似，命名为草假单胞菌RJJ-2 。RJJ-2菌株在35°C和pH 6.0的MSM中以红霉素（100 mg L ^-1）作为唯一碳源温育96小时后，降解了84.88％的红霉素。草酸青霉的最佳降解条件RJJ-2的温度为35°C，pH为6.0，并添加了0.1％的硫酸铵。HPLC-MS分析表明，主要的降解中间体为3-去吡喃糖基氧基红霉素A，cladinose，desosamine和7,12-dyhydroxy-6-deoxyerythronolideB。可以推断，红霉素被糖苷降解为3-depyranosyloxyerythromycinA。水解酶在初始反应中。