A close link between cardiovascular diseases and cancer results from sharing the same modifiable risk factors (e.g. nutritional) and cardiotoxicity of anti-cancerous therapies. It justifies cardio-oncological preliminary studies on dietary factors, especially on those of possible anti-carcinogenic or cardioprotective properties. The main purpose was to evaluate the effect of pomegranate seed oil (PSO) and/or bitter melon extract (BME) supplementation of the diet of female rats suffering from mammary tumors on lipidomic profile (expressed as fatty acids, conjugated fatty acids (CFA), malondialdehyde (MDA), cholesterol and oxysterols content) of cardiac tissue. Total lipidomic profile and intensity of lipid peroxidation in hearts of DMBA-treated Sprague-Dawley rats and their healthy equivalents, both obtaining diet supplementation, were evaluated with different chromatographic techniques coupled with appropriate detection systems (GC–MS, GC-TOFMS, Ag+-HPLC-DAD, UF-HPLC-DAD). Dietary modifications neither diminished breast cancer incidence nor exerted explicit cardio-protective influence, however, they diminished cholesterol content, i.a. because of inhibition of the endogenous conversion of squalene to cholesterol in cardiac tissue. CFA were incorporated into cardiac tissue to a lesser extent in the cancerous process. PSO and BME anti-oxidant properties in pathological condition were only slightly reflected in MDA levels but not in oxysterols formation. Obtained results indicate considerable changes in dietary supplements’ biological activity in pathological conditions and the need for clear distinction of drugs and dietary supplements, which is of utmost importance, especially for cancer survivors.

心血管疾病和癌症之间的密切联系是由于共享相同的可改变风险因素（例如营养）和抗癌疗法的心脏毒性。它证明了对饮食因素的心脏肿瘤学初步研究，特别是关于可能的抗癌或心脏保护特性的研究。主要目的是评估石榴籽油 (PSO) 和/或苦瓜提取物 (BME) 对患有乳腺肿瘤的雌性大鼠饮食的影响对脂质组学特征（以脂肪酸、共轭脂肪酸 (CFA) 表示） 、丙二醛 (MDA)、胆固醇和氧甾醇含量）的心脏组织。DMBA 治疗的 Sprague-Dawley 大鼠及其健康大鼠心脏中的总脂质组学特征和脂质过氧化强度，两者均获得饮食补充，使用不同的色谱技术和适当的检测系统（GC-MS、GC-TOFMS、Ag+-HPLC-DAD、UF-HPLC-DAD）进行评估。饮食调整既没有降低乳腺癌的发病率，也没有发挥明确的心脏保护作用，然而，它们降低了胆固醇含量，这是因为抑制了心脏组织中角鲨烯向胆固醇的内源性转化。在癌变过程中，CFA 在较小程度上并入心脏组织。病理状态下 PSO 和 BME 的抗氧化特性仅轻微反映在 MDA 水平上，而没有反映在氧甾醇形成中。获得的结果表明，病理条件下膳食补充剂的生物活性发生了相当大的变化，需要明确区分药物和膳食补充剂，这是最重要的，