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A ratiometric electrochemiluminescent cytosensor based on polyaniline hydrogel electrodes in spatially separated electrochemiluminescent systems
Analyst ( IF 3.6 ) Pub Date : 2021-1-15 , DOI: 10.1039/d0an02408b
Gen Liu 1, 2, 3, 4, 5 , Zixuan Chen 1, 2, 3, 4, 5 , Bao-Kang Jin 5, 6, 7, 8 , Li-Ping Jiang 1, 2, 3, 4, 5
Affiliation  

Here, we proposed a ratiometric electrochemiluminescent (ECL) strategy in spatially multiplied ECL systems. By the specific recognition of hyaluronic acid with proteoglycan CD44 and epidermal growth factor with epidermal growth factor receptor on the cell surface, the cells were labelled with potential-resolved ECL probes, namely Ru(bpy)32+ and g-C3N4, respectively. The as-proposed cytosensor provides a multichannel ECL protocol to improve the throughput, which may push the application of ECL for the cellular immunoanalysis.

中文翻译:

基于空间分离电化学发光系统中聚苯胺水凝胶电极的比例电化学发光细胞传感器

在这里,我们提出了空间倍增ECL系统中的比例电化学发光(ECL)策略。通过透明质酸与蛋白聚糖CD44和与细胞表面上的表皮生长因子受体的表皮生长因子的特异性识别,将细胞与潜在分辨ECL探针,即吡啶钌标记的3 2+和GC 3 Ñ 4分别。提出的细胞传感器提供了多通道ECL协议以提高通量,这可能推动ECL在细胞免疫分析中的应用。
更新日期:2021-01-27
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