In this study, spray deposition technology was used for in situ preparation of silver (Ag) particles on additively manufactured Ti6Al4V alloy surfaces. An amount of silver particles (200 nm) was uniformly achieved on pristine or acid-etched titanium (Ti) alloy surfaces by designing the spraying passes. Energy-dispersive X-ray spectroscopy and X-ray diffraction were used to confirm their composition and crystal structure. The antibacterial activity of the prepared silver particles was investigated against Staphylococcus aureus and Escherichia coli bacteria. Thereafter, the antibacterial activity of silver particles on Ti6Al4V alloy surfaces against S. aureus and E. coli bacteria was investigated by utilizing the bacterial counting method. The damaged cell membranes around the silver particles revealed excellent antibacterial properties, indicating the possibility of harnessing the spray deposition method to take advantage of the in-situ-produced antibacterial silver coatings on additively manufactured metallic materials. The excellent antibacterial activity of the surfaces suggests that silver particles prepared by spray deposition are potential candidates for future dental implant applications.

中文翻译：

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通过喷涂法在Ti6Al4V表面原位制备抗菌Ag颗粒

在这项研究中，喷涂技术被用于在增材制造的Ti6Al4V合金表面上原位制备银（Ag）颗粒。通过设计喷涂道次，可以在原始或酸蚀钛（Ti）合金表面上均匀地获得一定数量的银颗粒（200 nm）。能量色散X射线光谱学和X射线衍射被用来确认它们的组成和晶体结构。研究了制备的银颗粒对金黄色葡萄球菌和大肠杆菌的抗菌活性。此后，Ti6Al4V合金表面上的银颗粒对金黄色葡萄球菌和大肠杆菌的抗菌活性利用细菌计数方法研究细菌。银颗粒周围受损的细胞膜显示出优异的抗菌性能，这表明有可能利用喷雾沉积方法来利用增材制造的金属材料上原位生产的抗菌银涂层。表面优异的抗菌活性表明，通过喷涂沉积制备的银颗粒是未来牙科植入物应用的潜在候选者。