Background: After surgical correction of testicular torsion, up to 68% of ipsilateral testes undergo atrophy due to ischemia-reperfusion injury (IRI). Recent studies have shown that dexmedetomidine (Dex) alleviates IRI in various vital organs. However, those studies evaluated its protective effect on short-term reperfusion.
Purpose: We aimed to investigate whether Dex has a long-term protective effect against testicular injury after IRI.
Materials and Methods: A total of 24 New Zealand white rabbits were randomly divided into three groups (n = 8/group): the control group (saline-infused rabbits without IRI), the IRI group (saline-injected rabbits with IRI), and the Dex group (Dex-injected rabbits with IRI). The spermatic cord of rabbits in IRI and Dex groups was ligated for 4 h, and 1 h before reperfusion, Dex was administered intraperitoneally at a dose of 50 μg/kg body weight in group Dex, whereas saline was administered at the same dose to the IRI and control groups. Rabbits were kept alive for 4 weeks post reperfusion, then the testes were harvested, and the rabbits were euthanized.
Results: Four weeks post reperfusion, testicular volumes of the affected side decreased considerably in the IRI and Dex groups compared to the control group, with no significant difference between the IRI and Dex groups. Compared to the control group, the Johnson score and the mean seminiferous tubular diameters were significantly decreased in the IRI and Dex groups, but no significant differences were observed after administration of Dex. There were no significant differences in malondialdehyde and superoxide dismutase levels between the groups treated with and without Dex.
Conclusion: Dex administration 3 h after ischemia and 1 h before reperfusion did not demonstrate a significant protective effect against testicular injury 4 weeks after IRI in rabbits. Further research is needed to confirm the potential therapeutic effects of Dex by varying the experimental conditions.



中文翻译：

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给予右美托咪定对睾丸缺血再灌注损伤后的睾丸损伤没有长期保护作用

背景：睾丸扭转手术矫正后，高达 68% 的同侧睾丸因缺血再灌注损伤 (IRI) 而萎缩。最近的研究表明，右美托咪定 (Dex) 可减轻各种重要器官的 IRI。然而，这些研究评估了它对短期再灌注的保护作用。
目的：我们旨在调查 Dex 是否对 IRI 后的睾丸损伤具有长期保护作用。
材料和方法：新西兰大白兔24只，随机分为三组（n = 8/组）：对照组（无IRI的盐水注射兔）、IRI组（有IRI的盐水注射兔）和Dex组（带有 IRI 的 Dex 注射兔）。IRI组和Dex组结扎精索4 h，再灌注前1 h，Dex组腹腔注射Dex，剂量为50 μg/kg体重，对照组给予相同剂量的生理盐水。 IRI 和对照组。兔在再灌注后存活4周，然后收获睾丸，对兔实施安乐死。
结果：再灌注后4周，IRI和Dex组患侧睾丸体积与对照组相比显着减少，IRI和Dex组之间没有显着差异。与对照组相比，IRI和Dex组的Johnson评分和平均曲细精管直径显着降低，但Dex给药后没有观察到显着差异。在使用和未使用 Dex 治疗的组之间，丙二醛和超氧化物歧化酶水平没有显着差异。
结论：缺血后 3 小时和再灌注前 1 小时给予 Dex 对兔 IRI 后 4 周的睾丸损伤没有显着的保护作用。需要进一步研究以通过改变实验条件来确认 Dex 的潜在治疗效果。