ABSTRACT

BACKGROUND: Cognitive decline in the normal aging process is one of the most common and prominent problems. Delaying and alleviating cognitive impairment is an important strategy of anti-aging. This study is to aim at investigating the effects of Yinxing-Mihuan-Oral-Liquid(GMOL) on the CREB/BDNF signaling in the normal aging process.

METHODS: SD rats were randomly divided into GMOL group and control group. The Morris water maze (MWM) was introduced for behavioral test. Immunohistochemistry and immunofluorescence were used for cAMP response element binding protein 1(CREB1), p-CREB(Ser133), brain-derived neurotrophic factor(BDNF), synaptophysin(SYP) and glial fibrillary acidic protein(GFAP). Western blot was conducted for investigating the levels of CREB1 and p-CREB(Ser133), BDNF, SYP, GFAP and interleukin 6(IL-6). 

RESULTS: Our data showed that compared with the control group, GMOL group had higher expression of memory-related proteins, decreased inflammatory factors, and enhanced spatial learning and memory ability.

CONCLUSION: The study results show that GMOL ameliorates cognitive impairment of the normal aged SD rats via enhancing the expression of memory biomarkers and inhibiting inflammatory process. The potential neuroprotective role of GMOL in the process of aging may be related to mitigating cognitive decline via activating CREB/BDNF signaling and inhibiting inflammatory process.

摘要

背景：正常衰老过程中的认知能力下降是最常见和最突出的问题之一。延迟和减轻认知障碍是抗衰老的重要策略。本研究旨在研究银杏-米焕-口服液（GMOL）在正常衰老过程中对CREB ​​/ BDNF信号传导的影响。

方法： SD大鼠随机分为GMOL组和对照组。引入莫里斯水迷宫（MWM）进行行为测试。免疫组化和免疫荧光检测cAMP反应元件结合蛋白1（CREB1），p-CREB（Ser133），脑源性神经营养因子（BDNF），突触素（SYP）和神经胶质纤维酸性蛋白（GFAP）。进行了蛋白质印迹法以研究CREB1和p-CREB（Ser133），BDNF，SYP，GFAP和白介素6（IL-6）的水平。 

结果：我们的数据显示，与对照组相比，GMOL组的记忆相关蛋白表达更高，炎症因子减少，空间学习和记忆能力增强。

结论：研究结果表明，GMOL通过增强记忆生物标志物的表达和抑制炎症过程，减轻了正常老年SD大鼠的认知障碍。GMOL在衰老过程中的潜在神经保护作用可能与通过激活CREB ​​/ BDNF信号传导和抑制炎症过程减轻认知能力下降有关。