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Pressure-Based Biosensor Integrated with a Flexible Pressure Sensor and an Electrochromic Device for Visual Detection
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-01-25 , DOI: 10.1021/acs.analchem.0c04501 Zhenzhong Yu 1 , Guoneng Cai 1 , Xiaolong Liu 2 , Dianping Tang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-01-25 , DOI: 10.1021/acs.analchem.0c04501 Zhenzhong Yu 1 , Guoneng Cai 1 , Xiaolong Liu 2 , Dianping Tang 1
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This work demonstrated a pressure-based biosensor integrated with a flexible pressure sensor and an electrochromic device for visual detection. Initially, a sandwich-type immunoreaction for target carcinoembryonic antigen (CEA, as a model analyte) was carried out using the capture antibody (cAb) and platinum nanoparticles-labeled detection antibody (PtNPs-dAb) in a reaction cell. The added hydrogen peroxide (H2O2) could be catalyzed by the PtNPs to generate oxygen (O2). In a sealed chamber, the pressure increased with the overflowing O2. Meanwhile, a skin-inspired flexible pressure sensor with excellent sensing performance was fabricated to monitor the pressure change in real time. Thus, the electrical signal of the pressure sensor could reveal the target concentration. Moreover, a voltage-regulated electrochromic device based on polyaniline (PANI) and tungsten oxide (WO3) was integrated into the platform to provide a visualized readout. According to the electrical signal of the pressure sensor, the electrochromic device would change its color from green to blue, which also revealed the target concentration and could be observed by the naked eye. Under optimal conditions, the biosensor presented a high sensitivity for CEA in a detectable range of 0.2–50 ng/mL. The limit of detection (LOD) was 94 pg/mL. The selectivity, reproducibility, and accuracy were also satisfying. Furthermore, this immunoassay gives a path for developing visualized biosensors in point-of-care settings.
中文翻译：
基于压力的生物传感器，集成了柔性压力传感器和用于视觉检测的电致变色设备
这项工作演示了基于压力的生物传感器，该传感器集成了柔性压力传感器和用于视觉检测的电致变色设备。最初，在反应池中使用捕获抗体（cAb）和铂纳米颗粒标记的检测抗体（PtNPs-dAb）对目标癌胚抗原（CEA，作为模型分析物）进行了夹心型免疫反应。加入的过氧化氢（H 2 O 2）可以被PtNPs催化生成氧气（O 2）。在密闭室中，压力随着O 2的溢出而增加。同时，制造了具有出色传感性能的皮肤启发式柔性压力传感器，以实时监控压力变化。因此，压力传感器的电信号可以显示目标浓度。此外，基于聚苯胺（PANI）和氧化钨的调压电致变色器件（WO 3）集成到平台中以提供可视化的读数。根据压力传感器的电信号，电致变色设备会将其颜色从绿色更改为蓝色，这也揭示了目标浓度，并且可以用肉眼观察到。在最佳条件下，生物传感器对CEA的灵敏度很高，可检测范围为0.2–50 ng / mL。检测限（LOD）为94 pg / mL。选择性，重现性和准确性也令人满意。此外，该免疫测定法为在即时医疗点环境中开发可视化生物传感器提供了一条途径。
更新日期：2021-02-09
中文翻译：
基于压力的生物传感器，集成了柔性压力传感器和用于视觉检测的电致变色设备
这项工作演示了基于压力的生物传感器，该传感器集成了柔性压力传感器和用于视觉检测的电致变色设备。最初，在反应池中使用捕获抗体（cAb）和铂纳米颗粒标记的检测抗体（PtNPs-dAb）对目标癌胚抗原（CEA，作为模型分析物）进行了夹心型免疫反应。加入的过氧化氢（H 2 O 2）可以被PtNPs催化生成氧气（O 2）。在密闭室中，压力随着O 2的溢出而增加。同时，制造了具有出色传感性能的皮肤启发式柔性压力传感器，以实时监控压力变化。因此，压力传感器的电信号可以显示目标浓度。此外，基于聚苯胺（PANI）和氧化钨的调压电致变色器件（WO 3）集成到平台中以提供可视化的读数。根据压力传感器的电信号，电致变色设备会将其颜色从绿色更改为蓝色，这也揭示了目标浓度，并且可以用肉眼观察到。在最佳条件下，生物传感器对CEA的灵敏度很高，可检测范围为0.2–50 ng / mL。检测限（LOD）为94 pg / mL。选择性，重现性和准确性也令人满意。此外，该免疫测定法为在即时医疗点环境中开发可视化生物传感器提供了一条途径。
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