We asked whether transient Staphylococcus aureus in the oral environment synergistically interacts with orally associated bacterial species such as Actinomyces oris, Candida albicans, Fusobacterium nucleatum, Streptococcus oralis, Streptococcus mutans, and Veillonella dispar (six-species control biofilm 6S). For this purpose, four modified biofilms with seven species that contain either the wild type strain of the S. aureus genotype (USA300-MRSA WT), its isogenic mutant with MSCRAMM deficiency (USA300-MRSA ΔMSCRAMM), a methicillin-sensitive S. aureus (ST72-MSSA-) or a methicillin-resistant S. aureus (USA800-MRSA) grown on hydroxyapatite disks were examined. Culture analyses, confocal-laser-scanning microscopy and proteome analyses were performed. S. aureus strains affected the amount of supragingival biofilm-associated species differently. The deletion of MSCRAMM genes disrupted the growth of S. aureus and the distribution of S. mutans and S. oralis within the biofilms. In addition, S. aureus caused shifts in the number of detectable proteins of other species in the 6S biofilm. S. aureus (USA300-MRSA WT), aggregated together with early colonizers such as Actinomyces and streptococci, influenced the number of secondary colonizers such as Fusobacterium nucleatum and was involved in structuring the biofilm architecture that triggered the change from a homeostatic biofilm to a dysbiotic biofilm to the development of oral diseases.

中文翻译：

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金黄色葡萄球菌干扰链球菌空间分布和多种微生物口腔生物膜内物种的蛋白质表达


我们询问口腔环境中的短暂金黄色葡萄球菌是否与口腔相关细菌物种（如口放线菌、白色念珠菌、具核梭杆菌、口腔链球菌、变形链球菌和迪斯帕韦荣球菌（六种对照生物膜 6S））协同相互作用。为此，四个修饰的生物膜包含七个物种，其中包含金黄色葡萄球菌基因型的野生型菌株（USA300-MRSA WT）、具有MSCRAMM缺陷的同基因突变体（USA300-MRSA ΔMSCRAMM）、甲氧西林敏感的金黄色葡萄球菌(ST72-MSSA-) 或在羟基磷灰石盘上生长的耐甲氧西林金黄色葡萄球菌(USA800-MRSA) 进行了检查。进行了培养分析、共焦激光扫描显微镜和蛋白质组分析。金黄色葡萄球菌菌株对龈上生物膜相关物种的数量有不同的影响。 MSCRAMM 基因的缺失破坏了金黄色葡萄球菌的生长以及生物膜内变形链球菌和口腔链球菌的分布。此外，金黄色葡萄球菌引起 6S 生物膜中其他物种可检测蛋白质数量的变化。金黄色葡萄球菌(USA300-MRSA WT) 与放线菌和链球菌等早期定殖者聚集在一起，影响了具核梭杆菌等次级定殖者的数量，并参与构建生物膜结构，从而引发从稳态生物膜到菌群失调的变化生物膜促进口腔疾病的发展。